1. GEPAS Gene Expression Pattern Analysis Suite Ka-Lok Ng Dept. of Bioinformatics Asia University

2. Prepare a text file with tab delimiter Line starts with ‘#’ denotes comment http://gepas.bioinfo.cipf.es/ Tools  clustering  choose SOTA  upload the text file  decide the threshold, i.e. 80%  RUN To view the tree  click ‘send to SotaTree’  default parameters  RUN

3. Clusters information Click to view the profile

4. Profile – horiz. & vert. large scale Cluster 3

5. Diauxic data http://gepas3.bioinfo.cipf.es/cgi-bin/sets/diauxic

6. Diauxic data Tools  clustering  choose SOTA  upload the text file  decide the threshold, i.e. 90%  RUN To view the tree  click ‘send to SotaTree’  default parameters  RUN Click on the cluster 6, it has 108 elements

7. Extract cluster 6 - PlotCorr column 1 vs column 2 Threshold = 0.2 Threshold = 0.0

8. Extract cluster 6 – TreeView Tree width = 2200

9. Data – K-means cluster