1. BioQUEST 2012

2.  Case Study Format ◦ Learning Objectives ◦ Resources  Data Analysis & Visualization ◦ Tools ◦ Statistics  Assessment

3.  In this module, students will: ◦ (1) investigate the natural history of Vibrio cholerae, a pathogenic bacterium of global and historical significance. Use their knowledge of the organism’s biology to predict conditions favorable for epidemics. ◦ (2) compare and contrast cholera outbreaks from multiple locations. Determine whether demographic, environmental, or other variables are associated with an epidemic’s magnitude of impact. ◦ (3) model (or predict or explain) potential modes of disease transmission and/or dynamics of disease spread during an epidemic. ◦ (4) improve foundational skills in:  searching and obtaining data available online;  evaluating quality of datasets from a variety of sources; and  interpreting, analyzing, and visualizing diverse types of data

4. News & Media  http://www.catholic.org/international/international_story.php?id= 44401&utm_source=catholic.org%2Fhomepage&utm_medium=ba nner&utm_content=latest%2Bnews - Catholic Online, posted 1/13/12 http://www.catholic.org/international/international_story.php?id= 44401&utm_source=catholic.org%2Fhomepage&utm_medium=ba nner&utm_content=latest%2Bnews  http://www.nytimes.com/2012/01/10/health/haitian-cholera- epidemic-traced-to-first-known- victim.html?_r=1&scp=1&sq=cholera%20haiti&st=cse - discovery of first cholera case in Haiti, NYT 1/10/12 http://www.nytimes.com/2012/01/10/health/haitian-cholera- epidemic-traced-to-first-known- victim.html?_r=1&scp=1&sq=cholera%20haiti&st=cse  http://www.npr.org/templates/story/story.php?storyId=1449292 18 - discovery of first cholera case in Haiti http://www.npr.org/templates/story/story.php?storyId=1449292 18 Natural history, symptoms, epidemiology, general information  http://www.who.int/mediacentre/factsheets/fs107/en/index.html - WHO http://www.who.int/mediacentre/factsheets/fs107/en/index.html  http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001348/ - PubMed Health http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001348/  http://www.cdc.gov/cholera/general/ http://www.cdc.gov/cholera/general/  http://www.cdc.gov/haiticholera/ - CDC http://www.cdc.gov/haiticholera/

5. Accounts, reports of outbreaks  http://www.who.int/csr/don/archive/disease/cholera/en/index.ht ml - periodic updates about outbreaks globally http://www.who.int/csr/don/archive/disease/cholera/en/index.ht ml Profiles of Countries with Cholera  http://www.who.int/cholera/countries/en/index.html - general information (geographic, economic, environmental) for countries with cholera in their histories http://www.who.int/cholera/countries/en/index.html Data  http://apps.who.int/ghodata/?vid=2250 - cases, deaths, fatality rates by country, year http://apps.who.int/ghodata/?vid=2250  http://www.who.int/cholera/statistics/en/index.html - articles compiled annually with raw data http://www.who.int/cholera/statistics/en/index.html  http://www.who.int/gho/epidemic_diseases/cholera/en/index.ht ml - maps with data for cases, fatalities http://www.who.int/gho/epidemic_diseases/cholera/en/index.ht ml  http://emergency.cdc.gov/situationawareness/haiticholera/data.a sp - stratified by region and demographics http://emergency.cdc.gov/situationawareness/haiticholera/data.a sp

6. Maps  http://gamapserver.who.int/mapLibrary/app/searc hResults.aspx - WHO Cholera Map Library http://gamapserver.who.int/mapLibrary/app/searc hResults.aspx  http://emergency.cdc.gov/situationawareness/haiti cholera/staticmaps.asp - multiple static maps (distribution of outbreaks, cumulative attack rates, etc) http://emergency.cdc.gov/situationawareness/haiti cholera/staticmaps.asp  http://gamapserver.who.int/gho/interactive_charts /cholera/atlas.html - interactive http://new.paho.org/hq/images/Atlas_IHR/Cholera Hispaniola/atlas.html - interactive http://gamapserver.who.int/gho/interactive_charts /cholera/atlas.html http://new.paho.org/hq/images/Atlas_IHR/Cholera Hispaniola/atlas.html  http://www.bt.cdc.gov/situationawareness/haitich olera/Flash/bt/atlas.html - interactive, multiple filters and resolutions http://www.bt.cdc.gov/situationawareness/haitich olera/Flash/bt/atlas.html

7. http://books.google.com/books?id=LIYaAAAAMAAJ&lpg= PA173&ots=3A_xyGSTMb&dq=Cholera%20epidemic%20m ap%20of%20Nashville%2C%20Tennessee%2C%201873%20% 2F%20Ely%20McClellan.&pg=PP11#v=onepage&q&f=true

8. Cholera epidemic map of Nashville, Tennessee, 1873 / Ely McClellan. http://www.nlm.nih.gov/exhibition/cholera/images/a024226.jpg

11. http://www.worldmapper.org/posters/ worldmapper_map231_ver5.pdf

12. www.bit.ly/w6m9YO

13.  http://new.paho.org/hq/images/Atlas_IHR/C holeraHispaniola/atlas.html http://new.paho.org/hq/images/Atlas_IHR/C holeraHispaniola/atlas.html

14.  1) Finding the “RIGHT” parameters  a) Sanitation  b) Population Density  c) Environmental Conditions  d) Non availability of Clean water  e) Limited Access to health care  f) General Awareness of disease spread  etc……..

15.  Approach  1) Undergraduate Level  a) Study the data set  b) Descriptive statistics  c) Plot the data  d) Use Regression Approach  e) Find the optimal model  f) Run the model diagnostics

16.  Approach  2) Graduate Level  a) All above  b) Non-normal distribution  c) Poisson Modeling  c) MLE approach  d) Bayesian Model  e) Find the optimal model  f) Run the model diagnostics

17.  Pre/Post-Test Assessment ◦ Learning & Thinking Skills  Presentations, Activities, Group Work ◦ Science Process Skills  Solving problems, critical thinking, testing hypothesis ◦ Attitudes Toward Science  Comfort & confidence ◦ Module Content  Background info, mode of action & transmission, data analysis tools