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Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells Necrotic/Apoptotic Dead Treat Cells with DNAse to nick cells Incorporate FITC-UTP with.

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Presentation on theme: "Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells Necrotic/Apoptotic Dead Treat Cells with DNAse to nick cells Incorporate FITC-UTP with."— Presentation transcript:

1 Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells Necrotic/Apoptotic Dead Treat Cells with DNAse to nick cells Incorporate FITC-UTP with TdT Apoptotic Live Fix cells and permeabilize Incorporate B-UTP with TdT then PE-Avidin

2 10 01234 TDT48015 FITC UTP -> 02565127681024 TDT48015 FSC -> SIMULTANEOUS EVALUATION OF DEAD, NECROTIC AND APOPTOTIC CELLS

3 R4 R1R2 R3 PE-dUTP Fl-12-dUTP A FSC SSC gated R1 B FSC SSC gated R3 C FSC SSC gated R2 D LIGHT SCATTER ANALYSIS OF DEAD CELLS AND VIABLE APOPTOTIC CELLS

4 FOUR COLOR DETECTION OF SURFACE ANTIGENS, APOPTOSIS AND DEAD CELLS PE-CD19 DEAD CELLS TC-CD33APOPTOSIS

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