1. 1 April, 2005 Chapter C4.1 and C5.1 DNA Microarrays and Cancer

2. Overview DNA microarrays allow the researcher to assay the transcription of a number of genes simultaneously. This technique is especially informative regarding large- scale changes in gene expression. Global analysis of gene expression is of particular interest in the understanding of cancer - a disease of gene expression. Cancer cell type and progression status can be probed using mmicroarray methods.

4. Differentially labeled cDNA sets: green = control, red = experimental

5. Hybridization

6. Microarray Data

7. Microarray and Northern Data

8. Clustering of Microarray Data

9. Global Shift in Gene Expression

11. DLBCL Microarray Experiment

12. Clustering in the DLBCL Experiment

13. Discovery of DLBCL Subtypes

14. Clinical DLBCL Outcomes

15. Expression Changes in Breast Cancer

16. Cluster Analysis

17. Microarray Detection of Genomic Rearrangements

18. Southern Confirmation