1. © 2013 Pearson Education, Inc. Chapter 9: Biotechnology and DNA Technology $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Biotechnology Tools of Biotechnology Recombinant DNA Applications of rDNA Products of Genetic Engineering FINAL ROUND

2. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $100 Question Today, chemicals that organisms do NOT naturally make are made by microorganisms and a. plants. b. fungi. c. protozoa. d. helminths. ANSWER BACK TO GAME

3. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $100 Answer Today, chemicals that organisms do NOT naturally make are made by microorganisms and a. plants. b. fungi. c. protozoa. d. helminths. BACK TO GAME

4. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $200 Question When E. coli has the ability to produce the hormone insulin, what is the reason? a. It picked up the insulin gene from another cell. b. It is an ancient gene that now has no function. c. The insulin gene was inserted into it. d. It needs to regulate its cell glucose level. BACK TO GAME ANSWER

5. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $200 Answer When E. coli has the ability to produce the hormone insulin, what is the reason? a. It picked up the insulin gene from another cell. b. It is an ancient gene that now has no function. c. The insulin gene was inserted into it. d. It needs to regulate its cell glucose level. BACK TO GAME

6. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $300 Question Natural selection is the process a.of inserting genes into cells by rDNA technology. b.whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits. c.by which scientists select desirable breeds of animals or strains of plants to cultivate. d.None of the answers is correct. BACK TO GAME ANSWER

7. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $300 Answer Natural selection is the process a.of inserting genes into cells by rDNA technology. b.whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits. c.by which scientists select desirable breeds of animals or strains of plants to cultivate. d.None of the answers is correct. BACK TO GAME

8. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $400 Question In the field of genetic engineering, cDNA is important because it a. lacks exons. b. lacks introns. c. is really RNA. d. is really reverse transcriptase. BACK TO GAME ANSWER

9. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $400 Answer In the field of genetic engineering, cDNA is important because it a. lacks exons. b. lacks introns. c. is really RNA. d. is really reverse transcriptase. BACK TO GAME

10. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $500 Question The procedure during which cells can take up DNA from the surrounding environment is called a. electroporation. b. protoplast fusion. c. transformation. d. microinjection. BACK TO GAME ANSWER

11. © 2013 Pearson Education, Inc. Topic 1: Biotechnology $500 Answer The procedure during which cells can take up DNA from the surrounding environment is called a. electroporation. b. protoplast fusion. c. transformation. d. microinjection. BACK TO GAME

12. © 2013 Pearson Education, Inc. Which technique amplifies small samples of DNA to quantities that are large enough for analysis? a. hybridization b. PCR c. RFLP d. DNA fingerprinting Topic 2: Tools of Biotechnology $100 Question BACK TO GAME ANSWER

13. © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $100 Answer Which technique amplifies small samples of DNA to quantities that are large enough for analysis? a. hybridization b. PCR c. RFLP d. DNA fingerprinting BACK TO GAME

14. © 2013 Pearson Education, Inc. Which of the following techniques is used to identify bacteria carrying a specific gene? a. cloning b. Western blot c. transformation d. DNA probe Topic 2: Tools of Biotechnology $200 Question BACK TO GAME ANSWER

15. © 2013 Pearson Education, Inc. Which of the following techniques is used to identify bacteria carrying a specific gene? a. cloning b. Western blot c. transformation d. DNA probe Topic 2: Tools of Biotechnology $200 Answer BACK TO GAME

16. © 2013 Pearson Education, Inc. If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends. a. flat b. sticky c. adhesive d. blunt Topic 2: Tools of Biotechnology $300 Question BACK TO GAME ANSWER

17. © 2013 Pearson Education, Inc. If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends. a. flat b. sticky c. adhesive d. blunt Topic 2: Tools of Biotechnology $300 Answer BACK TO GAME

18. © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $400 Question BACK TO GAME ANSWER A population of genetically identical cells carrying a desired vector is called a. a clone. b. a species. c. a library. d. PCR.

19. © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $400 Answer BACK TO GAME A population of genetically identical cells carrying a desired vector is called a. a clone. b. a species. c. a library. d. PCR.

20. © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $500 Question Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells? a. protoplast fusion b. viral transduction c. transformation d. cloning BACK TO GAME ANSWER

21. © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $500 Answer Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells? a. protoplast fusion b. viral transduction c. transformation d. cloning BACK TO GAME

22. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $100 Question Which of the following is NOT a method for inserting foreign DNA into cells? a. transformation b. microinjection c. restriction digestion d. electroporation BACK TO GAME ANSWER

23. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $100 Answer Which of the following is NOT a method for inserting foreign DNA into cells? a. transformation b. microinjection c. restriction digestion d. electroporation BACK TO GAME

24. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $200 Question Which of the following is a eukaryote used in genetic engineering? a. Agrobacterium tumefaciens b. Pseudomonas aeruginosa c. Saccharomyces cerevisiae d. Thermus aquaticus BACK TO GAME ANSWER

25. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $200 Answer Which of the following is a eukaryote used in genetic engineering? a. Agrobacterium tumefaciens b. Pseudomonas aeruginosa c. Saccharomyces cerevisiae d. Thermus aquaticus BACK TO GAME

26. © 2013 Pearson Education, Inc. What are the two main sources of DNA that scientists use to obtain the genes in which they are interested? a. genomic libraries and PCR b. synthetic DNA and plasmids c. synthetic DNA and genomic libraries d. PCR and plasmids Topic 3: Recombinant DNA $300 Question BACK TO GAME ANSWER

27. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $300 Answer What are the two main sources of DNA that scientists use to obtain the genes in which they are interested? a. genomic libraries and PCR b. synthetic DNA and plasmids c. synthetic DNA and genomic libraries d. PCR and plasmids BACK TO GAME

28. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $400 Question Restriction enzymes are a. animal enzymes that splice RNA. b. bacterial enzymes that destroy phage DNA. c. animal enzymes that destroy RNA. d. viral enzymes that destroy host DNA. BACK TO GAME ANSWER

29. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $400 Answer BACK TO GAME Restriction enzymes are a.animal enzymes that splice RNA. b.bacterial enzymes that destroy phage DNA. c.animal enzymes that destroy RNA. d.viral enzymes that destroy host DNA.

30. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $500 Question Which enzyme is used to synthesize cDNA? a. RNA polymerase b. complementary polymerase c. reverse transcriptase d. DNA polymerase BACK TO GAME ANSWER

31. © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $500 Answer Which enzyme is used to synthesize cDNA? a. RNA polymerase b. complementary polymerase c. reverse transcriptase d. DNA polymerase BACK TO GAME

32. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $100 Question In plants, the Ti plasmid can cause a. crown gall disease. b. tobacco mosaic disease. c. Pseudomonas. d. soft rot disease. BACK TO GAME ANSWER

33. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $100 Answer In plants, the Ti plasmid can cause a. crown gall disease. b. tobacco mosaic disease. c. Pseudomonas. d. soft rot disease. BACK TO GAME

34. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $200 Question Molecule-sized robots used to detect contamination in food are an example of a. forensic microbiology. b. nanotechnology. c. biotechnology. d. bioinformatics. ANSWER BACK TO GAME

35. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $200 Answer Molecule-sized robots used to detect contamination in food are an example of a. forensic microbiology. b. nanotechnology. c. biotechnology. d. bioinformatics. BACK TO GAME

36. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $300 Question What can be used to screen a sample for multiple pathogens simultaneously? a. DNA chips b. Southern blotting c. shotgun sequencing d. colony hybridization BACK TO GAME ANSWER

37. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $300 Answer What can be used to screen a sample for multiple pathogens simultaneously? a. DNA chips b. Southern blotting c. shotgun sequencing d. colony hybridization BACK TO GAME

38. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $400 Question When mammalian cells are used to make protein products in genetic engineering, there is a(n) a. high risk of toxin production. b. increase in allergic reactions. c. low risk of toxin production. d. low rate of protein production. BACK TO GAME ANSWER

39. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $400 Answer BACK TO GAME When mammalian cells are used to make protein products in genetic engineering, there is a(n) a. high risk of toxin production. b. increase in allergic reactions. c. low risk of toxin production. d. low rate of protein production.

40. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $500 Question Which bacterium has been genetically modified to produce a human protein, gamma interferon? a. Bacillus thuringiensis b. Staphylococcus aureus c. Escherichia coli d. Pseudomonas fluorescens BACK TO GAME ANSWER

41. © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $500 Answer Which bacterium has been genetically modified to produce a human protein, gamma interferon? a. Bacillus thuringiensis b. Staphylococcus aureus c. Escherichia coli d. Pseudomonas fluorescens BACK TO GAME

42. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $100 Question The Ti plasmid naturally occurs in a. Thermus aquaticus. b. Saccharomyces cerevisiae. c. Bacillus thuringiensis. d. Agrobacterium tumefaciens. BACK TO GAME ANSWER

43. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $100 Answer The Ti plasmid naturally occurs in a. Thermus aquaticus. b. Saccharomyces cerevisiae. c. Bacillus thuringiensis. d. Agrobacterium tumefaciens. BACK TO GAME

44. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $200 Question The method of using RFLPs to identify bacterial or viral pathogens is called a. proteomics. b. DNA fingerprinting. c. genetic screening. d. DNA sequencing. BACK TO GAME ANSWER

45. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $200 Answer The method of using RFLPs to identify bacterial or viral pathogens is called a. proteomics. b. DNA fingerprinting. c. genetic screening. d. DNA sequencing. BACK TO GAME

46. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $300 Question One of the earliest commercial products of rDNA technology was a. rennin. b. cellulase. c. malaria vaccine. d. human growth hormone. BACK TO GAME ANSWER

47. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $300 Answer BACK TO GAME One of the earliest commercial products of rDNA technology was a. rennin. b. cellulase. c. malaria vaccine. d. human growth hormone.

48. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $400 Question Which of the following is NOT a therapeutic application of biotechnology? a. creating herbicide-resistant plants b. producing DNA vaccines c. gene therapy d. synthesizing subunit vaccines BACK TO GAME ANSWER

49. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $400 Answer Which of the following is NOT a therapeutic application of biotechnology? a. creating herbicide-resistant plants b. producing DNA vaccines c. gene therapy d. synthesizing subunit vaccines BACK TO GAME

50. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $500 Question If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is a. inserting the Ti into Agrobacterium. b. transformation of an animal cell. c. transformation of E. coli with Ti. d. splicing Ti into a plasmid. BACK TO GAME ANSWER

51. © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $500 Answer If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is a. inserting the Ti into Agrobacterium. b. transformation of an animal cell. c. transformation of E. coli with Ti. d. splicing Ti into a plasmid. BACK TO GAME

52. © 2013 Pearson Education, Inc. FINAL ROUND Question One surprising finding in the Human Genome Project was that “junk DNA,” which does NOT code for a functional product, makes up what percentage of the human genome? a. 90% b. 95% c. 98% d. 85% BACK TO GAME ANSWER

53. © 2013 Pearson Education, Inc. FINAL ROUND Answer One surprising finding in the Human Genome Project was that “junk DNA,” which does NOT code for a functional product, makes up what percentage of the human genome? a. 90% b. 95% c. 98% d. 85% BACK TO GAME