1. 13-1 Nuclear Magnetic Resonance Spectroscopy Part-3 Prepared By Dr. Khalid Ahmad Shadid Islamic University in Madinah Department of Chemistry

2. 13-2 Spin-Spin Splitting in 1 H NMR Spectra Peaks are often split into multiple peaks due to interactions between nonequivalent protons on adjacent carbons, called spin-spin splitting (multiplicity or coupling pattern) Remember that a proton will exist in two spin states and Simple coupling patterns (obey the n+1 rule, see later) protons are only coupled to one other set of neighboring protons or protons are coupled to multiple sets of neighboring protons by identical coupling constants If the neighbours are not all equivalent, more complex patterns

3. 13-3 In the absence of any other hydrogen atoms, Ha (ABOVE) has a conventional resonance frequency/chemical shift that is determined by its local magnetic field as usual Spin-Spin Splitting in 1 H NMR Spectra The PRESENCE OF Hb, however, alters the local magnetic field at Ha, the TWO possible spin states at Hb results in both a small INCREASE in the local field (alpha spin) and also a small DECREASE in the local field (beta spin), this results in TWO NEW resonance frequencies/chemical shifts, the peak is split into TWO, it becomes a DOUBLET

4. 13-4 The presence of the TWO hydrogens HB (BELOW) result in THREE local magnetic fields at proton Ha, the peak for HA is similarly split into THREE, it appears as a triplet. Spin-Spin Splitting in 1 H NMR Spectra

5. 13-5 We will discuss only splitting occurs with protons on ADJACENT carbon atoms. The area ratio's of the peaks in a splitting pattern is given by Pascal's triangle Spin-Spin Splitting in 1 H NMR Spectra

6. 13-6 Signal Splitting; the (n + 1) Rule  Peak:  Peak: The units into which an NMR signal is split; singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m) and so forth.  Signal splitting:  Signal splitting: Splitting of an NMR signal into a set of peaks by the influence of neighboring nonequivalent hydrogens.  (n + 1) rule:  (n + 1) rule: If a hydrogen has n hydrogens nonequivalent to it but equivalent among themselves on the same or adjacent atom(s), its 1 H-NMR signal is split into (n + 1) peaks.

7. 13-7 Signal Splitting (n + 1)  Figure 13.12 1 H-NMR spectrum of 1,1-dichloroethane.

8. 13-8 Origins of Signal Splitting  Signal coupling:  Signal coupling: An interaction in which the nuclear spins of adjacent atoms influence each other and lead to the splitting of NMR signals.  Coupling constant (J) :  Coupling constant (J) : The separation on an NMR spectrum (in hertz) between adjacent peaks in a multiplet.  A quantitative measure of the spin-spin coupling with adjacent nuclei.

9. 13-9 Origins of Signal Splitting  Figure 13.13 Vicinal H atoms and the spin-spin coupling that occurs between them.  H atoms with more than three bonds between them generally do not exhibit noticeable coupling.  For H atoms three bonds apart, the coupling is referred to as vicinal coupling.

10. 13-10 Origins of Signal Splitting  Figure 13.15 The quartet-triplet 1 H-NMR signals of 3- pentanone showing the original trace and a scale expansion to show the signal splitting more clearly.

11. 13-11 Spin-Spin Splitting: Simple  Example: 1,1-dichloroethane

12. 13-12 Coupling in 1 H NMR Spectra  Coupling arises because the magnetic field of vicinal protons influences the field that the proton experiences  Consider: effect of -CH group on the adjacent -CH 3  The methine -CH can adopt two alignments and as a result, the signal for the adjacent methyl -CH 3 is split in two lines, of equal intensity, a doublet

13. 13-13  Consider: effect of -CH 3 group on the adjacent -CH  The methyl -CH 3 protons give rise to 8 possible combinations with respect to the applied field  Some combinations are equivalent and there are four magnetically different effects  As a result, the signal for the adjacent methine -CH is split into four lines, of intensity ratio 1:3:3:1, a quartet Coupling in 1 H NMR Spectra

14. 13-14 Simple Spin-Spin Splitting  An adjacent CH 3 group can have four different spin alignments as 1:3:3:1  This gives peaks in ratio of the adjacent H signal  An adjacent CH 2 gives a ratio of 1:2:1  The separation of peaks in a multiplet is measured is a constant, in Hz  J (coupling constant)

15. 13-15 Spin-Spin Splitting Patterns  The relative intensities, given by Pascal's triangle, are due to interactions between nuclear spins that can have two possible alignments with respect to the magnetic field  To derive Pascal's triangle, start at the apex, and generate each lower row by creating each number by adding the two numbers above and to either side in the row above together

16. 13-16 Rules for Spin-Spin Splitting  Equivalent protons do not split each other  The signal of a proton with n equivalent neighboring H’s is split into n + 1 peaks  Protons that are farther than two carbon atoms apart do not split each other

17. 13-17 17 Molecules With Two Type of Protons  How many signals?

18. 13-18  Two groups of protons coupled to each other have the same coupling constant, J  J coupling constant is the distance between peaks in a multiplet (in Hz)  Peaks for protons that split each other will always have the same coupling constant  J useful in determining which peaks are adjacent to each other  Multiplets will often be skewed in the direction of the peak to which they are coupled Rules for Spin-Spin Splitting

19. 13-19  For the peaks of bromoethane, the coupling constant J is 7 Hz  J calculation: take the distance (in ppm) between any two adjacent split peaks and multiply it by the frequency of the NMR machine Coupling Constant

20. 13-20  How to distinguish between A and B? Example

21. 13-21 Example

22. 13-22  Coupling between two nuclei can be:  Homonuclear: between same nuclei (e.g. 1 H-C-C- 1 H)  Heteronuclear: between different nuclei (e.g. 1 H- 13 C)  Factors affecting spin-spin couplings : the nuclei involved, the distance between the two nuclei, the angle of interaction between the two nuclei, and the nuclear spin of the nuclei  Distance Dependence Basics of Nuclear Coupling one-bond ( 1 J) > two-bond > ( 2 J) three-bond ( 3 J) > long-range ( 4 J - n J) 110 - 270 Hz 9 - 15 Hz 6 - 8 Hz 1 - 7 Hz

23. 13-23  Geminal coupling or two-bond coupling ( 2 J)  Dependent upon the bond angle between the nuclei Generally, the smaller the angle the bigger the coupling constant Basics of Nuclear Coupling

24. 13-24  Vicinal coupling or three-bond coupling ( 3 J)  Dependent upon the dihedral angle between the nuclei. Generally, the more eclipsed or antiperiplanar the nuclei the greater the coupling constant Basics of Nuclear Coupling

25. 13-25 25 Complex Patterns of Proton Coupling  The n+1 rule fails when protons in a group are magnetically nonequivalent and may have different chemical shifts and coupling constants  Spectra can be more complex due to overlapping signals, multiple nonequivalence  Example: trans-cinnamaldehyde

26. 13-26 Complex Patterns of Proton Coupling

27. 13-27 Complex Patterns of Proton Coupling  Doublet-of-doublets! Hb is split by Ha with a J of around 15 Hz. It is further split by proton Hc with a J of around 10 Hz. One way that you know it is not a quartet is that the peaks are all (roughly) the same height  Because of the small coupling between Ha and Hc, one of the splittings is very small, and consequently is difficult to see in the real spectrum

28. 13-28 Coupling of Protons on Oxygen  Normally, no coupling is observed between the O-H and H’s on the carbon atom to which the hydroxyl group is attached  The average time of residence of a proton on oxygen is 10-5 seconds. About 10-2 to 10-3 second is required for an NMR transition event to be recorded. Thus, the hydroxyl proton is unattached more frequently than it is attached to oxygen, and the spin interaction between the hydroxyl proton and any other protons is effectively decoupled  Likewise, protons on nitrogen also exchange quickly enough to be uncoupled from other protons in the molecule

29. 13-29 Uses of 1 H NMR Spectroscopy  The technique is used to identify likely products in the laboratory quickly and easily  Example: regiochemistry of hydroboration/oxidation of methylenecyclohexane  Only that for cyclohexylmethanol is observed

30. 13-30 13 C NMR Spectroscopy

31. 13-31 13 C NMR Spectroscopy: Background  Like 1 H, 13 C has I = ½.  For 12 C, I = 0, no NMR signal observed  13 C has only about 1.1% natural abundance. As a result, C is about 400 times less sensitive than H nucleus to the NMR phenomena  13 C resonances are 0 to 220 ppm downfield from TMS  Similar factors affect the chemical shifts in 13 C as seen for 1 H NMR  Long relaxation times (excited state to ground state) mean no integrations

32. 13-32 Characteristics of 13 C NMR Spectroscopy  Provides a count of the different types of environments of carbon atoms in a molecule  Number of peaks indicates the number of types of C  Spectrum of 2-butanone is illustrative: signal for C=O carbons on left edge

33. 13-33 Proton-Coupled 13 C Spectra  Due to low natural abundance of 13 C (1.1%), any 13 C is unlikely to have another 13 C nucleus as a neighbour in natural material. Therefore:  we do not usually see 13 C- 13 C coupling in “normal” 13 C spectra  13 C– 1 H couplings are observed in “normal” 13 C spectra unless the interactions are “decoupled”  As a result H’s split the carbon peaks into multiplets

34. 13-34 34 Proton-Broad Band Decoupled 13 C Spectra  13 C Broad Band Decoupled (BBD) spectra are normally obtained as proton-decoupled spectra to remove all interaction between 13 C nuclei and protons  In the proton-decoupled spectra, each carbon atom gives a singlet  Proton-decoupled spectra are much easier to interpret but it dose not indicate the number of hydrogen atoms attached to each carbon atom  From BBD spectrum you know:  number of chemically nonequivalent carbons, and  chemical shift (environment of each type of carbon)

35. 13-35 13 C-NMR Spectroscopy  Figure 13.29 Proton-decoupled 13 C-NMR spectrum of 1- bromobutane.

36. 13-36 How would you determine the nature of the carbon?  The good news is (as you know): both 13 C and 1 H have nuclear spin quantum numbers I = 1/2  Therefore: coupling (and J) can be observe between 13 C and 1 H  Spin-spin splitting between 13 C and 1 H follows the same rules as 1 H– 1 H splitting  The N+1 Rule can be applied: singletdoublet tripletquartet

37. 13-37 Proton-Coupled Viz Proton-Decoupled 13 C Spectra  Example: ethyl phenylacetate

38. 13-38 13 C NMR Chemical Shifts  Similar factors affect (electronegativity, hybridization, anisotropy) the chemical shifts in 13 C as seen for 1 H NMR  Since C is more electronegative than H, A 13 C atom linked to more C atoms and therefore fewer 1H atoms will be deshielded & is at a lower field (i.e. higher  value) a) Saturated C 8 - 60 ppm b) C attached to electronegative atoms 40 - 70 ppm c) Alkene/ aromatic C100-150 ppm d) Carbonyl carbons155-220 ppm

39. 13-39 39 Simplified 13 C Correlation Charts

40. 13-40 Chemical Shift - 13 C-NMR  Figure 13.30 13 C-NMR chemical shifts of representative groups.

41. 13-41 Chemical Shift - 13 C-NMR

42. 13-42 Interpreting NMR Spectra  Alkanes  1 H-NMR signals appear in the range of  0.8-1.7.  13 C-NMR signals appear in the considerably wider range of  10-60.  Alkenes  1 H-NMR signals appear in the range  4.6-5.7.  1 H-NMR coupling constants are generally larger for trans-vinylic hydrogens (J= 11-18 Hz) compared with cis-vinylic hydrogens (J= 5-10 Hz).  13 C-NMR signals for sp 2 hybridized carbons appear in the range  100-160, which is to higher frequency from the signals of sp 3 hybridized carbons.

43. 13-43 Interpreting NMR Spectra  Figure 13.31 1 H-NMR spectrum of vinyl acetate.

44. 13-44 Interpreting NMR Spectra  Alcohols  1 H-NMR O-H chemical shift often appears in the range  3.0-4.0, but may be as low as  0.5.  1 H-NMR chemical shifts of hydrogens on the carbon bearing the -OH group are deshielded by the electron- withdrawing inductive effect of the oxygen and appear in the range  3.0-4.0.  Ethers  A distinctive feature in the 1 H-NMR spectra of ethers is the chemical shift,  3.3-4.0, of hydrogens on the carbons bonded to the ether oxygen.

45. 13-45 Interpreting NMR Spectra  Figure 13.32 1 H-NMR spectrum of 1-propanol.

46. 13-46 Interpreting NMR Spectra  Aldehydes and ketones  1 H-NMR: aldehyde hydrogens appear at  9.5-10.1.  1 H-NMR:  -hydrogens of aldehydes and ketones appear at  2.2-2.6.  13 C-NMR: carbonyl carbons appear at  180-215.  Amines  1 H-NMR: amine hydrogens appear at  0.5-5.0 depending on conditions.

47. 13-47 Interpreting NMR Spectra  Carboxylic acids  1 H-NMR: carboxyl hydrogens appear at  10-13, to higher frequency of most other types of hydrogens.  13 C-NMR: carboxyl carbons in acids and esters appear at  160-180. Figure 13.33 1 H-NMR spectrum if 2-methylpropanoic acid.

48. 13-48 Interpreting NMR Spectra  Spectral Problem 1; molecular formula C 5 H 10 O.

49. 13-49 Interpreting NMR Spectra  Spectral Problem 2; molecular formula C 7 H 14 O.

50. 13-50 Problem 13.21  Following is the 1 H-NMR spectrum of compound O, molecular formula C 7 H 12. Compound O reacts with bromine in carbon tetrachloride to give a compound with the molecular formula C 7 H 12 Br 2. The 13 C-NMR spectrum of compound O shows signals at  150.12,106.43, 35.44, 28.36, and 26.36. Deduce the structural formula of O.

51. 13-51 Problem 13.21  The molecular formula, C 7 H 12, indicates a hydrogen deficiency of 2, so there are two rings and/or pi bonds. Compound O reacts with only one mole of bromine, so there is only one double bond and there then must be one ring. Furthermore, the 13C-NMR shows only two sp 2 resonances,another evidence that there is only one double bond.

52. 13-52 Good Luck With thanks to Dr. Ziad Ali, Taibah University for providing NMR resources