1. AMPLIFCATION-REFRACTORY MUTATION SYSTEM (ARMS-PCR) IN SCD MOLECULAR DIAGNOSIS INTRODUCTION TO ARMS-PCR

2. EXON 1EXON 2EXON 3 6 A. EXON 1EXON 2EXON 3 6B. gAg cTc gTg cAc A T amplicon common ARMS-PCR IN SCD DIAGNOSIS

3. EXON 1EXON 2EXON 3 6 EXON 1EXON 2EXON 3 6 A. gAg cTc gTg cAc T A B. common ARMS-PCR cont.

4. GEL ELECTROPHORESIS

5. GEL ELECTROPHORESIS cont.

6. 6 EXON 1EXON 2 gAg cTc control amplicon  A allele amplicon A Outer 2 A Outer 1 “usual” “altered”  A ALLELE TETRA-ARMS

7. 6 EXON 1EXON 2 gTg cAc A control amplicon Outer 2 A Outer 1  S allele amplicon “usual” “altered”  A ALLELE TETRA-ARMS

8. TETRA-ARMS PCR ELCTROPHORESIS