1. rough- Endoplasmatic Reticulum rER Sortingofproteins László KŐHIDAI, Med. Habil. MD.,PhD. Assoc. Professor Department of Genetics, Cell- and Immunobiology Semmelweis University Sept 29/ 2014

2. Endoplasmic = inside the cell; reticulum = network Extensive membrane system Extensive membrane system Includes up to half of membrane of cell Includes up to half of membrane of cell Tubules and sacs = cisternae Tubules and sacs = cisternae Continous with the nuclear envelope Continous with the nuclear envelope Two types: rough ER (ribosomes) Two types: rough ER (ribosomes) smooth ER smooth ER

3. rER rER

4. s-ER (smooth ER) Structure: tubular Function: synthesis of phospholipids, cholesterol, synthesis of phospholipids, cholesterol, ceramide ceramide synthesis of steroids synthesis of steroids storage and regulation of Ca 2+ storage and regulation of Ca 2+ detoxification – cyt P450 detoxification – cyt P450

5. TEM of ribosomes attached to the rER in a pancreatic exocrine cell

6. mRNA peptide polyribosome

7. Ribosomes – mRNA – Polyribosome

8. Molecular composition of ribosome 60S rRNA + peptides rRNA

9. Ribosome subunits

12. Comparison of prokaryotic and eukaryotic ribosomes

13. Structure of ribosome ?

14. t-RNAactivatorenzyme of AA of AA ribosome anticodon codon

15. Initiation

16. Elongation

23. Peptide bond formation peptidyl transferase peptide bond

24. Termination

26. Internalization of peptides into the rER

28. Synthesis of secretory proteins on the rER

29. Structure of SRP Universal Universal 300 base RNA 300 base RNA Six proteins Six proteins P54 - signal peptide P54 - signal peptide P9, P14 - ribosome P9, P14 - ribosome P68, P72 move the P68, P72 move the peptide peptide

30. Synthesis of secretory proteins on the rER

32. Electron microscopic view of a translocon channel

33. The ribosome-translocon-ER membrane complex

34. Translocon complex TRAM – (= translocating chain-associated membrane TRAM – (= translocating chain-associated membrane protein) binds the signal sequence Sec61p – major constituent of the translocon channel; Sec61p – major constituent of the translocon channel; assembles into a donut-like structure assembles into a donut-like structure The Sec 61 complex binds the ribosome, The Sec 61 complex binds the ribosome, participates the transmembrane transfer

35. Cycles of GDP/GTDP exchange and GTP hydrolysis that drive insertion of nascent secretory protein into the translocon

36. Topologies of some integral membrane proteins synthesized on the rER

39. Synthesis and insertion into the ER membrane of the insulin receptor and similar proteins N-terminus faces to ER lumen C-terminus faces to cytosol C-terminus faces to cytosol A signal sequence is cleaved A signal sequence is cleaved Stop-transfer membrane-anchor Stop-transfer membrane-anchor signal signal

40. Synthesis and insertion into the ER membrane of the asialoglycoprotein receptor and similar proteins C-terminus faces to ER lumen C-terminus faces to ER lumen N-terminus faces to cytosol N-terminus faces to cytosol No N-terminal ER signal sequence No N-terminal ER signal sequence an uncleaved integral signal an uncleaved integral signal membrane-anchor sequence membrane-anchor sequence

41. Synthesis and insertion into the ER membrane of proteins with multiple transmembrane  -helical segments - An uncleaved internal signal membrane-anchor sequence - A stop-transfer membrane-anchor sequence - An uncleaved internal signal membrane-anchor sequence Etc. Etc.

42. Post-translational modification Proteolytic cleavage of proteins Proteolytic cleavage of proteins Glycosilation Glycosilation Acylation Acylation Methylation Methylation Phosphorylation Phosphorylation Sulfation Sulfation Prenylation Prenylation Vitamin C-dependent modifications Vitamin C-dependent modifications Vitamin K-dependent modifications Vitamin K-dependent modifications Selenoproteins Selenoproteins

45. Proteolytic cleavage Removal of signal peptide from Removal of signal peptide from preproproteins preproproteinspreproteins Signal peptidase Signal peptidase

46. Properties of uptake-targeting signal sequences Target organelle Usual signal location within protein Signal removal Nature of signal rERN-terminal+ „core” of 6-12 mostly hydrophobic amino acids, often proceeded by one or more basic amino acids MitochondriumN-terminal+ 3-5 nonconsecutive Arg or Lys residues often with Ser and Thr; no Glu or Asp ChloroplastN-terminal+ No common motives, generally rich in Ser,Thr, poor in Glu and Asp PerixisomeC-terminal-Ser-Lys-Leu NucleusInternal- Cluster of 5 basic amino acids or two samller clusters separated by 10 amino acids

47. Glycoproteins Predominant sugars are: glucose, galactose, mannose, fucose, GalNAc, GlcNAc, NANA GalNAc, GlcNAc, NANA O-glycosidic linkage – hydroxyl group of Ser, Thr, hydrLys N-glycosidic linkage – consensus sequence N-X-S(T) (BUT No P) Major N-linked families: high mannose type, hybride type, complex type (sialic acids)

48. GlycosilationrER N-linkage to GlcNAc rER O-linkage to GalNAc

49. O-linked sugars: sugars coupled to UDP, GDP (mannose), CMP (NANA) glycosprotein glycosylttransferase N-linked sugars: Requires a lipid intermediate dolichol phosphate

50. N-Glycosilation

52. Glycosylphosphatodyl inositol (GPI) -anchored peptides GPI-anchored peptides become the outer surface of the surface membrane

53. Protein folding: Protein Disulfide Isomerase (PDI) Provides mechanism Provides mechanism for breaking incorrectly for breaking incorrectly paired disulfide bonds. paired disulfide bonds. The most stable folded The most stable folded sate is reached sate is reached

54. Protein folding: Peptidyl-prolyl isomerase: Peptidyl-prolyl isomerase: accelerates rotation about peptidyl-prolyl bonds Oligosaccharide protein transferase: Oligosaccharide protein transferase: transfers carbohydrate chains to the nascent polypeptide as they enter the lumen of ER Calnexin, calreticulin: Calnexin, calreticulin: interact with CHO groups of glycoproteins

55. Protein signals: Integral, soluble proteins of ER, Golgi retrieved by Integral, soluble proteins of ER, Golgi retrieved by the KDEL-receptors. They recognize the KDEL signal the KDEL-receptors. They recognize the KDEL signal (Lys-Asp-Glu-Leu at C-terminus). (Lys-Asp-Glu-Leu at C-terminus). ER membrane proteins have a KKXX (dilysine motif) ER membrane proteins have a KKXX (dilysine motif) on the C-terminus. on the C-terminus. Other ER membrane proteins possess di-arginine motif Other ER membrane proteins possess di-arginine motif on the N-terminus. on the N-terminus.

56. Chase-pulse technique

57. Antibiotics They inhibit different steps of protein synthesis Actinomycin D- transcription (complex with DNA) Actinomycin D- transcription (complex with DNA) Rifamycin- transcription (RNA polymerase) Rifamycin- transcription (RNA polymerase) Amanitin- transcription (RNA polymerase II) Amanitin- transcription (RNA polymerase II) Streptomycin- iniciation Streptomycin- iniciation Tetracycline- aminoacyl-tRNA - A locus interaction Tetracycline- aminoacyl-tRNA - A locus interaction Erythromycin- translocation of tRNA from A to P locus Erythromycin- translocation of tRNA from A to P locus Cycloheximide- “ (only in eukaryotes) Cycloheximide- “ (only in eukaryotes) Chloramphenicol- peptide bond formation Chloramphenicol- peptide bond formation Puromycin- termination Puromycin- termination Penicillins and Cephalosporins - synthesis of bacterial cell wall (proteoglycans) (proteoglycans)

58. AAPA actinomycinrifamycinamanitin streptomycin tetracycline chloramphenicol erythromycin, cycloheximide puromycin