1. Free Response Questions

2. Warm-up
Create a flow chart to show how the following words are related to each other: allosteric regulation enzyme protein structure structure and function

3. 1. Discuss the lock-and-key theory of enzyme- substrate interaction giving a specific example to illustrate the theory. Include in your discussion the effects of each of the following: a. Substrate concentration b. pH shifts c. Temperature shifts d. Competitive inhibition

4. 1. Answer
-Explanation of lock-and-key fit -Specific example given -Substrate concentration: more substrate = more binding at active site of enzyme = more reactants per unit time. Saturation point is the point at which more substrate does not increase reaction rate -pH and temperatures can denature enzymes -denaturation = change in structure, which changes active binding site -competitive inhibition site- it competes w/substrate to bind to enzyme

5. 2. Describe the chemical composition and configuration of enzymes and discuss the factors that modify enzyme structure and/or function

6. 2. Answer
-enzymes are catalytic proteins; thus, are made of amino acids -enzymes have active binding cites that are specific to the substrate shape -Enzymes function in lowering energy to activation, and the shape of the active binding site must fit the substrate -Factors that modify enzyme structure, each one must be described: -changes in pH and temperature -competitive inhibitors prevent substrate binding, and thus affect its function. They bind on the binding site -noncompetitive inhibition; bind on enzyme, but not at active site4; affect function -coenzymes/cofactors: non-enzyme factors that bind to the enzyme to give it its functional shape so that substrates can bind to the active site

7. 3.
You have 15 minutes to finish this.

8. Enzyme video

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