1. Chapter 3: Observing Microorganisms Through a Microscope
Preparing Smears for Staining
Compound Microscope
Electron Microscopy
Microscopy
Staining
$100
$100
$100
$100
$100
$200
$200
$200
$200
$200
$300
$300
$300
$300
$300
$400
$400
$400
$400
$400
$500
$500
$500
$500
$500
FINAL ROUND

2. Topic 1: Compound Microscope $100 Question
magnifies the specimen
focuses light through the specimen
holds the microscope slide in position
controls the amount of light entering the condenser
What is the function of the diaphragm on a compound microscope?
Answer = D
ANSWER
BACK TO GAME

3. Topic 1: Compound Microscope $100 Answer
magnifies the specimen
focuses light through the specimen
holds the microscope slide in position
controls the amount of light entering the condenser
What is the function of the diaphragm on a compound microscope?
BACK TO GAME

4. Topic 1: Compound Microscope $200 Question
The ocular lens is used to
magnify the image from the objective lens.
improve resolution.
increase the light.
decrease the refractive index.
Answer = A
ANSWER
BACK TO GAME

5. Topic 1: Compound Microscope $200 Answer
The ocular lens is used to
magnify the image from the objective lens.
improve resolution.
increase the light.
decrease the refractive index.
BACK TO GAME

6. Topic 1: Compound Microscope $300 Question
Brightfield illumination is produced by the
condenser.
objective.
lens.
annular diaphragm.
Answer = A
ANSWER
BACK TO GAME

7. Topic 1: Compound Microscope $300 Answer
Brightfield illumination is produced by the
condenser.
objective.
lens.
annular diaphragm.
BACK TO GAME

8. Topic 1: Compound Microscope $400 Question
Most ocular lenses magnify specimens by a factor of
100.
10.
1000.
2000.
Answer = B
ANSWER
BACK TO GAME

9. Topic 1: Compound Microscope $400 Answer
Most ocular lenses magnify specimens by a factor of
100.
10.
1000.
2000.
BACK TO GAME

10. Topic 1: Compound Microscope $500 Question
The ability of the lenses to distinguish fine detail and structure is called
illumination.
magnification.
refractive index.
resolution.
Answer = D
ANSWER
BACK TO GAME

11. Topic 1: Compound Microscope $500 Answer
The ability of the lenses to distinguish fine detail and structure is called
illumination.
magnification.
refractive index.
resolution.
BACK TO GAME

12. Topic 2: Microscopy $100 Question
Which microscope would you choose to view the cellular contents of a microbe in its natural state?
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
Answer = B
ANSWER
BACK TO GAME

13. Topic 2: Microscopy $100 Answer
Which microscope would you choose to view the cellular contents of a microbe in its natural state?
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
BACK TO GAME

14. Topic 2: Microscopy $200 Question
Which microscope uses an ultraviolet light source?
compound light microscope
phase-contrast microscope
fluorescence microscope
electron microscope
Answer = C
ANSWER
BACK TO GAME

15. Topic 2: Microscopy $200 Answer
Which microscope uses an ultraviolet light source?
compound light microscope
phase-contrast microscope
fluorescence microscope
electron microscope
BACK TO GAME

16. Topic 2: Microscopy $300 Question
Which microscope achieves the highest magnification and the greatest resolution?
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
Answer =D
ANSWER
BACK TO GAME

17. Topic 2: Microscopy $300 Answer
Which microscope achieves the highest magnification and the greatest resolution?
compound light microscope
phase-contrast microscope
darkfield microscope
electron microscope
BACK TO GAME

18. Topic 2: Microscopy $400 Question
Which microscope is especially useful in studying the surface structures of intact cells and viruses?
transmission electron microscope
scanning electron microscope
phase-contrast microscope
darkfield microscope
Answer = B
ANSWER
BACK TO GAME

19. Topic 2: Microscopy $400 Answer
Which microscope is especially useful in studying the surface structures of intact cells and viruses?
transmission electron microscope
scanning electron microscope
phase-contrast microscope
darkfield microscope
BACK TO GAME

20. Topic 2: Microscopy $500 Question
What microscope should be used to view three-dimensional images?
differential interference contrast microscope
darkfield microscope
phase-contrast microscope
transmission electron microscope
Answer = A
ANSWER
BACK TO GAME

21. Topic 2: Microscopy $500 Answer
What microscope should be used to view three-dimensional images?
differential interference contrast microscope
darkfield microscope
phase-contrast microscope
transmission electron microscope
BACK TO GAME

22. Topic 3: Electron Microscopy $100 Question
Objects smaller than about 0.2 micrometers, such as viruses, must be examined with a(n)
compound microscope.
electron microscope.
phase-contrast microscope.
simple microscope.
Answer = B
ANSWER
BACK TO GAME

23. Topic 3: Electron Microscopy $100 Answer
Objects smaller than about 0.2 micrometers, such as viruses, must be examined with a(n)
compound microscope.
electron microscope.
phase-contrast microscope.
simple microscope.
BACK TO GAME

24. Topic 3: Electron Microscopy $200 Question
In electron microscopy, the specimen is placed on a
copper mesh grid.
glass slide.
plastic slide.
magnesium mesh grid.
Answer = A
ANSWER
BACK TO GAME

25. Topic 3: Electron Microscopy $200 Answer
In electron microscopy, the specimen is placed on a
copper mesh grid.
glass slide.
plastic slide.
magnesium mesh grid.
BACK TO GAME

26. Topic 3: Electron Microscopy $300 Question
for the study of the very smallest specimens, such as virus particles.
to provide a general idea of size and shape of the specimen.
to increase the electron opacity of the surrounding field.
to enhance the contrast between the specimen and the background.
Shadow casting is useful
Answer = B
ANSWER
BACK TO GAME

27. Topic 3: Electron Microscopy $300 Answer
Shadow casting is useful
for the study of the very smallest specimens, such as virus particles.
to provide a general idea of size and shape of the specimen.
to increase the electron opacity of the surrounding field.
to enhance the contrast between the specimen and the background.
BACK TO GAME

28. Topic 3: Electron Microscopy $400 Question
In a scanning electron microscope, objects are generally magnified
100 to 100,000x.
100 to 10,000x.
1000 to 100,000x.
1000 to 10,000x.
Answer = D
ANSWER
BACK TO GAME

29. Topic 3: Electron Microscopy $400 Answer
In a scanning electron microscope, objects are generally magnified
100 to 100,000x.
100 to 10,000x.
1000 to 100,000x.
1000 to 10,000x.
BACK TO GAME

30. Topic 3: Electron Microscopy $500 Question
In a transmission electron microscope, objects are generally magnified
1000 to 100,000x.
1000 to 10,000x.
10,000 to 100,000x.
10,000 to 1,000,000x.
Answer = C
ANSWER
BACK TO GAME

31. Topic 3: Electron Microscopy $500 Answer
In a transmission electron microscope, objects are generally magnified
1000 to 100,000x.
1000 to 10,000x.
10,000 to 100,000x.
10,000 to 1,000,000x.
BACK TO GAME

32. Topic 4: Preparing Smears for Staining $100 Question
to kill and color the microorganisms
to color the microorganisms and fix them to the slide
to kill the microorganisms and fix them to the slide
to kill and color the microorganisms and fix them to the slide
Why are smear preparations fixed?
Answer = C
ANSWER
BACK TO GAME

33. Topic 4: Preparing Smears for Staining $100 Answer
Why are smear preparations fixed?
to kill and color the microorganisms
to color the microorganisms and fix them to the slide
to kill the microorganisms and fix them to the slide
to kill and color the microorganisms and fix them to the slide
BACK TO GAME

34. Topic 4: Preparing Smears for Staining $200 Question
Basic dyes are _________ ions and are _________ to most types of bacteria.
positive; attracted
negative; attracted
positive; not attracted
negative; not attracted
Answer = A
ANSWER
BACK TO GAME

35. Topic 4: Preparing Smears for Staining $200 Answer
Basic dyes are _________ ions and are _________ to most types of bacteria.
positive; attracted
negative; attracted
positive; not attracted
negative; not attracted
BACK TO GAME

36. Topic 4: Preparing Smears for Staining $300 Question
What is staining?
observing shapes, sizes, and capsules of microorganisms
killing the microorganisms and fixing them to the slide
spreading a thin film of material containing microorganisms on a slide
coloring the microorganisms with a dye that emphasizes certain structures
Answer = D
ANSWER
BACK TO GAME

37. Topic 4: Preparing Smears for Staining $300 Answer
What is staining?
observing shapes, sizes, and capsules of microorganisms
killing the microorganisms and fixing them to the slide
spreading a thin film of material containing microorganisms on a slide
coloring the microorganisms with a dye that emphasizes certain structures
BACK TO GAME

38. Topic 4: Preparing Smears for Staining $400 Question
To stain an organism correctly, one must first
kill the organism.
stain the slide.
prepare a smear.
fix the slide.
Answer = C
ANSWER
BACK TO GAME

39. Topic 4: Preparing Smears for Staining $400 Answer
To stain an organism correctly, one must first
kill the organism.
stain the slide.
prepare a smear.
fix the slide.
BACK TO GAME

40. Topic 4: Preparing Smears for Staining $500 Question
Distortions of cell size and shape are minimized in
negative staining.
Gram staining.
acid-fast staining.
endospore staining.
Answer = A
ANSWER
BACK TO GAME

41. Topic 4: Preparing Smears for Staining $500 Answer
Distortions of cell size and shape are minimized in
negative staining.
Gram staining.
acid-fast staining.
endospore staining.
BACK TO GAME

42. Topic 5: Staining $100 Question
What is the mordant in the Gram stain?
iodine
safranin
alcohol-acetone
crystal violet
Answer = A
ANSWER
BACK TO GAME

43. Topic 5: Staining $100 Answer
What is the mordant in the Gram stain?
iodine
safranin
alcohol-acetone
crystal violet
BACK TO GAME

44. Topic 5: Staining $200 Question
Flagella are stained with a(n)
carbolfuchsin stain.
malachite green stain.
methylene blue stain.
acid-fast stain.
Answer = A
ANSWER
BACK TO GAME

45. Topic 5: Staining $200 Answer
Flagella are stained with a(n)
carbolfuchsin stain.
malachite green stain.
methylene blue stain.
acid-fast stain.
BACK TO GAME

46. Topic 5: Staining $300 Question
Capsules are stained with a(n)
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
Answer = A
ANSWER
BACK TO GAME

47. Topic 5: Staining $300 Answer
Capsules are stained with a(n)
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
BACK TO GAME

48. Topic 5: Staining $400 Question
Endospores are stained with a(n)
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
Answer = C
ANSWER
BACK TO GAME

49. Topic 5: Staining $400 Answer
Endospores are stained with a(n)
negative stain.
Gram stain.
Schaeffer-Fulton stain.
acid-fast stain.
BACK TO GAME

50. Topic 5: Staining $500 Question
What color are gram-negative bacteria at the completion of the Gram staining process?
pink
purple
colorless
brown
Answer = A
ANSWER
BACK TO GAME

51. Topic 5: Staining $500 Answer
What color are gram-negative bacteria at the completion of the Gram staining process?
pink
purple
colorless
brown
BACK TO GAME

52. FINAL ROUND Question
The appearance of Mycobacterium leprae after they are stained with an acid-fast stain is
purple.
red.
colorless.
brown.
Answer = B
ANSWER
BACK TO GAME

53. FINAL ROUND Answer
The appearance of Mycobacterium leprae after they are stained with an acid-fast stain is
purple.
red.
colorless.
brown.
BACK TO GAME