1. Phage Nanobiotechnology Tatiana Samoylova Phages – ideal naturally occurring nanomaterials phages - viruses that infect bacteria exhibit a great diversity of shapes in nanometer range (examples below) well-defined geometry and uniformity favorable for nanofabrication phage coat proteins can be engineered using simple recombinant DNA techniques Phage Lambda, http://textbookofbacteriology.net/phage_2. html Filamentous M13 phage, EM image. T. Samoylova Segment of ~1% of filamentous landscape phage particle. Phage coat proteins are grey. Engineered fusion peptides are black. V. Petrenko.

2. Phage Nanobiotechnology Tatiana Samoylova 7 nm 900 nm Phage display technology set of phage-based techniques for identification of target-specific peptides or proteins targets include biomolecules, whole cells, whole tissues in living organisms, non-biological surfaces (for example, metals) phage display libraries are mixtures of distinct phage clones filamentous phage is predominant phage display vector library consists of billions of phage clones – billions opportunities to select phage clones specific to desired targets pIII pVI pVIII pVII + pIX

3. Phage Nanobiotechnology Tatiana Samoylova pIII pVI pVIII pVII + pIX 7 nm g8 900 nm Phage display technology set of phage-based techniques for identification of target-specific peptides or proteins targets include biomolecules, whole cells, whole tissues in living organisms, non-biological surfaces (for example, metals) phage display libraries are mixtures of distinct phage clones filamentous phage is predominant phage display vector library consists of billions of phage clones – billions opportunities to select phage clones specific to desired targets

4. Phage Nanobiotechnology Tatiana Samoylova What we are doing with phage nanomaterials (current focus) whole phage particles as vaccines for contraception of animals (cat/dog/pig) phage particles that display peptides with contraceptive properties -GnRH peptide, zona-pellucida binding peptides -phage body – carrier protein, peptides – antigens phage is effective immunogen (nanosize, architecture), low cost, stable, safe shown below – sperm-oocyte interaction in in vitro fertilization experiment in the presence of (A) normal serum or (B) serum from immunized animal Normal porcine serum Porcine anti-serum, contains anti- sperm antibodies A B

5. Phage Nanobiotechnology Tatiana Samoylova What others at AU are doing with phage nanomaterials targeted liposomes with phage fusion proteins for cancer-specific drug/gene delivery (V Petrenko, CVM, illustration below) devices for pathogen detection using filamentous phage (Bryan Chin, AU Detection and Food Safety Center) devices for detection of bacterial infection using lytic phage (Aleksandr Simonian, College of Engineering) fabrication of phage-based biosensors (V Vodyanoy, CVM) magnetic nanoparticles targeted with phage-derived peptides for imaging of brain tumors (A David, College of Engineering, illustration on the right) + Landscape phage coat protein units displaying tumor- specific peptide Therapeutic liposome containing drugs Tumor-targeted liposome Multi-domain cores 100 nm Magnetic nanoparticles targeted to brain tumor with phage-derived peptides

6. Phage Nanobiotechnology Tatiana Samoylova Phage nanomaterials in medicine and technology: applications affinity matrix for purification of biomolecules scaffold for tissue engineering/repair medical diagnostics and monitoring molecular imaging targeted drug and gene delivery vaccine development detection of pathogens and biothreats in microelectronics, as template for fabrication of nanowires and nanocomposite films details in this book New hires for AU bioinformatics computational proteomics systems biology (systeomics)