1. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

2. Label-free quantitative proteomics using qTOF MS Sample, Tissue, Plasma Yeast Enolase (100 fmol) lyse Individual proteins

3. lyse Thousands of proteins Trypsin digest all proteins to peptides Tens/ hundreds of thousands of peptides QTOF Sequence everything Sample Label-free quantitative proteomics using qTOF MS

4. Time Amount of peptide (larger the response, more protein present)

5. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

6. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

7. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

8. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

9. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

10. UCL Institute of Child Health & Great Ormond Street Hospital for Sick Children UCL

11. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

12. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

13. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

14. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

15. Acknowledgements UCL