1. Synthetic Biology Crash Course DAY ONE

2. Pre-Assessment 1:00-1:10

3. Who We Are

4. Course Schedule Day One -Overview of Key Biology Concepts -Overview of Lab Protocols Day Two -Lab Activities: Creating a BioBrick Day Three -Modelling Workshop -Ethics Workshop

5. Day One Schedule 1:00-1:15 - Introduction and Pre-Assessment 1:15-1:45 - General Biology Refresher 1:45-2:15 - Introduction to Synthetic Biology 2:15-2:30 - Micropipetting Exercise

6. What Is Synthetic Biology? “...The design and construction of new biological entities such as enzymes, genetic circuits, and cells or the redesign of existing biological systems.” Synberc (Synthetic Biology Engineering Research Center)

7. DNA and Genes: The Basis of Synthetic Biology 1:15 – 1:45

8. DNA and RNA

9. DNA at Work Replication Transcription Translation

10. Genes

11. Gene Constructs

12. Plasmids

13. BioBrick Formation 1:45-2:15

14. Isolating a Gene Polymerase Chain ReactionPolymerase Chain Reaction (PCR)

15. Restriction Enzyme Digest

16. Gel Electrophoresis

17. Ligation Reaction

18. Transformation Reaction

19. Confirmation

20. Activity: Micropipetting 2:15-2:30

21. Homework ●Read the following selections from the “vGEM Protocol Handbook” ○ Restriction Enzyme Digest ○ Gel Electrophoresis ○ Transformation and Plating ●Explore the iGEM Parts Registry: http://parts.igem.org/Main_Page