1. Accepting samples from NICHD investigators for Next Generation sequencing

2.  MGL will cover 2/3 of sequencing costs.  Investigator will have to pay their share at time of award.  Applications will be evaluated by a committee.

3. ABI SOLiD 5500xlIon Torrent PGM

4.  2 FlowChips, 6 lanes each  Up to 1.8 billion fragments per run  Up to 200 Gigabases per run  Each run takes about 3 weeks

5.  1 Chip  Up to 5 million fragments per run  Up to 2 Gigabases per run  Each run takes about 7 hours Ideal for:  Microbiome  Targeted exome

6.  Whole exome (3)  Targeted exome (1)  RNA-Seq (4)  Microbiome (2)  Methylome (1)  Ribosomal profiling (1)  ChIP-Seq (1)  MicroRNA-Seq (1)

7.  Final Award meeting in two weeks  Select members of committee  Discuss final details  Applications due Sunday April 5  Committee decisions Friday April 17  Final Award announcements April 25

8. Application process (April-May 2014; 8-member scientific review committee)  Number of Applications: 18 (18 PIs)  Number of Programs: 7  (PDEGEN[5], PGD[5], OSD[3], PRAE[1], MMP[1], PDN[1], PPD[1])  Total funding requested: $346,000  * Number of Applications Funded: 9 (9 PIs; 4 Programs)  * Total awarded: $150,000

9. MGL is looking to fill the position of laboratory supervisor See the OITE or DDIR websites for details

10. Website: mgl.nichd.nih.gov List serv: MGL-USERS-L Email: NICHDDIRMolGenomicsLab@mail.nih.govMolGenomicsLab@mail.nih.gov Phone: 301-402-4563 Walk-in: Bldg 10/Rm 9D41