1. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Lipidomics

2. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Same principle as the proteomic mass spectral method Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer  lipids separated by UPC 2 chromatography (supercritical fluid chromatography)  Uses CO 2 to separate lipids out according to their polar properties not non-polar (reverse phase)  Lipids are easier to identify than metabolites as they have been more extensively studied C24 C20 C18 C16 C14 C12 C10 C8 C22 Free fatty acids

3. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Same principle as the proteomic mass spectral method Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer  lipids separated by UPC 2 chromatography (supercritical fluid chromatography)  Uses CO 2 to separate lipids out according to their polar properties not non-polar (reverse phase)  Lipids are easier to identify than metabolites as they have been more extensively studied Triacylglycerols Chosteryl esters ( Mixture of FFA, TAG, and CE

4. Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Phospholipid analyses from human heart Sphingomyelin Phosphatidylglycerol ceramides Phosphatidylethanolamine Lyso-phosphatidyl- ethanolamine Phosphatidylcholine Lyso phosphatidyl choline Phosphatidylcholine C34:0 C36:0 C38:3 C36:1 C36:3 C32:0 C30:1