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Aim: How can we measure area on ImageJ?
Day 2
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Why use area? Useful in colony counts Area of growth
Zone of inhibition Can measure multiple areas simultaneously
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How to measure area (single shape)
Set Scale Analyze Decide which tool to use- rectangle, ellipse, polygon (best choice!) Measure Record value for area
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How to measure area (color adjustment)
This is only for Black and White photos! (We will do color tomorrow) Set scale Rectangle tool over scale Right click; clear Image Type 8-bit Process Noise Despeckle (2-3x)
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How to measure area (color adjustment)
Process Binary Make binary Make sure entire shape is one color (despeckle if not) Analyze Analyze particles Show Outlines Check “Display Results”
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Compare original to outline
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Assignment Junior High Researchers
Calculate area using polygon AND Color Adjustment for Image B and Image D Senior High Researchers Calculate area using polygon AND color Adjustment for Image C and E
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