1. Aim: How can we measure area on ImageJ?
Day 2

2. Why use area? Useful in colony counts Area of growth
Zone of inhibition
Can measure multiple areas simultaneously

3. How to measure area (single shape)
Set Scale
Analyze
Decide which tool to use- rectangle, ellipse, polygon (best choice!)
Measure
Record value for area

4. How to measure area (color adjustment)
This is only for Black and White photos! (We will do color tomorrow)
Set scale
Rectangle tool over scale
Right click; clear
Image
Type
8-bit
Process
Noise
Despeckle (2-3x)

5. How to measure area (color adjustment)
Process
Binary
Make binary
Make sure entire shape is one color (despeckle if not)
Analyze
Analyze particles
Show
Outlines
Check “Display Results”

6. Compare original to outline

7. Assignment Junior High Researchers
Calculate area using polygon AND Color Adjustment for Image B and Image D
Senior High Researchers
Calculate area using polygon AND color Adjustment for Image C and E