1. Tubular Organs

2. Tracheal Development DT LT DB

3. Dysfusion (Dys) is a bHLH-PAS Protein bHLHPAS-1PAS-2

4. Dysfusion Is Expressed In Fusion Cells DT DB LT Btl-GAL4, UAS-lacZ Dys

5. dys Mutants dys 3 dys 2 bHLHPAS-1PAS-2 Mab 2A12 WTdys

6. How dys expression is regulated? How Dys:Tgo regulates down-stream target genes?

7. Dysfusion is Expressed in Mutiple Cell Types

8. Identify dys Enhancer

9. Identify dys Fusion Cell Enhancer

10. How dys expression is regulated? Is dys auto-regulated by Dys:Tgo? N283-GFP,dys/Tm3, twi-gal4, UAS-GFP C1.7-GFP, dys/TM3, twi-gal4, UAS-GFP Is dys regulated by esg? esg/cyo, twi-gal4, UAS-GFP; N283 esg/cyo, twi-gal4, UAS-GFP; C1.7 Identify other transcription factors binding sites in dys enhancer. Identify dys fusion cells enhancer in C1.7 and G1.7 Ongoing……

11. Summary of dys Function N TC GTG members only (Protein trafficking) Trachealess (Migration) CG13196, E-cadherin (cell adhesion) CG15252 (unknown function) Tgo Dys Tgo Dys Tgo Dys Identified dys Targets

12. Two TCGTG Sites are Required to Activates CG13196

13. Two TCGTG Sites of CG13196 are Conserved

14. Two TCGTG Sites are Required to Activates CG15252

15. Two TCGTG Sites of CG15252 are Conserved

16. Potential Co-activator of Dys:Tgo is Required to Turn on Gene Expression in Additional Fusion Cells

17. Ongoing..