1. BWBmin Administrative Web Interface for Paracel BioView WorkBench Frances Tong Marc Rieffel, PhD Paracel Southern California Bioinformatics Summer Institute Summer 2004 Funded by the National Science Foundation and the National Institutes of Health

2. Overview Paracel products –Paracel BLAST Software for BLAST searching –GeneMatcher Supercomputer for genetic algorithms (Smith-Waterman, etc) –BioView Workbench Graphical interface for user to run Paracel BLAST and algorithms on GeneMatcher. Goal : Develop a user-friendly web interface for administration of BioView Workbench

3. BioView Workbench : Graphical User Interface for Paracel BLAST and GeneMatcher Searches Search submission interface which lets you access Paracel algorithms without entering the command-line environment

4. Administrative Tasks for BioView Workbench Database management : – loading new databases – modifying existing databases – deleting existing databases – creating new database sets – adding new members to sets – removing members from sets – deleting entire sets General Configuration : – setting of general system parameters Each requires command line interface and/or manual editing of specific files

5. BWBmin : User-friendly alternative Web interface for admin tasks Highly awaited by biologists Built from scratch in 9 weeks as a Webmin module Currently consists of 7 CGI scripts written in Perl, HTML and Javascript, a Perl module and other setup files

6. Before BWBmin… command line usage Ex: loading a new database (UCSC Human Chromosome 11) into Paracel BLAST and GeneMatcher adding to Paracel BLAST adding to GeneMatcher still not done yet ! …

7. ~ open and edit database configuration file for BioView Workbench Beginning of file… Go to the end of the file and type in database information according to the format: Hash table : ‘Key’ => { name => ‘-------’, type => ‘----’, seqcount => ‘----’, date => ‘-----’, btk => ‘--------’, btkoracle => ‘-------’, pb => ‘-------’, };

8. a web-based interface for system administration for Unix developed by Jamie Cameron anyone can develop and distribute their own Webmin modules for any purpose open source at http://webmin.com/download.html

9. Advantages of BWBmin Automates and makes admin tasks for Paracel BLAST and GeneMatcher more user friendly and faster –Elimination of multiple steps –No dealing with the syntax errors that may result with the command line interface and manual editing of configuration files

10. General Configuration

11. Database Management

12. Loading Databases Load fasta file as Paracel BLAST formatted database Load fasta file as GeneMatcher formatted database Load fasta file as GeneMatcher formatted query Input New Database Information

13. Modifying Databases

14. Deleting Databases Selectively remove databases

15. Loading Existing Databases onto BWB

16. Creating Database Sets

17. Removing Members from Database Sets

18. Future Work Construction of similar pages for managing: – Paracel Filtering Package – Preloaded queries – Score matrices Building of release for clients

19. Acknowledgements Paracel Dr. Marc Rieffel Dave Meyer Peter Wilson Jun Qian Mike Curtin SoCalBSI Dr. Jamil Momand Dr. Nancy Warter-Perez Dr. Sandra Sharp Dr. Wendie Johnston Jackie Heras Fellow Interns National Science Foundation : National Institutes of Health