1. Protein Structure

2. Why study protein structure? Studying the structure model allows better understanding of the structure-function relationship, and is an important starting point for many kinds of research

3. Structure determination Crystallography: A solution of protein molecules is assembled into a periodic lattice A solution of protein molecules is assembled into a periodic lattice The crystal is bombarded with X-ray beams The crystal is bombarded with X-ray beams The collision of the beams with the atom electrons creates a diffraction pattern The collision of the beams with the atom electrons creates a diffraction pattern The diffraction pattern is transformed into an electron density map of the protein from which the 3D locations of the atoms can be deduced The diffraction pattern is transformed into an electron density map of the protein from which the 3D locations of the atoms can be deduced F F

4. Structure determination Nuclear magnetic resonance (NMR): A solution of the protein is placed in a magnetic field A solution of the protein is placed in a magnetic field Spins align parallel or Spins align parallel or anti-parallel to the field RF pulses of electromagnetic energy shift spins from their alignment RF pulses of electromagnetic energy shift spins from their alignment Upon radiation termination spins re- align while emitting the energy they absorbed Upon radiation termination spins re- align while emitting the energy they absorbed The emission spectrum contains information about the identity of the nuclei and their immediate environment The emission spectrum contains information about the identity of the nuclei and their immediate environment The result is an ensemble of models rather than a single one The result is an ensemble of models rather than a single one

5. PDB: Protein Data Bank http://www.rcsb.org

6. PDB model  Defines the 3D coordinates (x,y,z) of each of the atoms in one or more molecules (i.e., complex)  There are models of proteins, protein complexes, proteins and DNA, protein segments, etc  The models also include the positions of ligand molecules, solvent molecules, metal ions, etc  PDB ID: integer + 3 integers/characters (e.g., 1a14)

7. The PDB file format

8. ATOM Records: Usually protein or DNA HETATM Records: Usually Ligand, ion, water chain Residue identity Residue number Atom number Atom identity atom coordinates Temperature factor XYZ Occupancy

10. Background and motivation  DNA methylation at DNA CpG sites has a central role in imprinting (plants and mammals), but it is not clear how the imprinting machinery recognizes its target genes  The Dnmt3 protein family (a,b,l) are de novo methyltransferases  Dnmt3a and Dnmt3l KO mice show altered sex- specific de novo methylation in germ cells, indicating that these proteins are both required for the methylation of most imprinted loci in germ cells Goal: Conduct a structural and biochemical study of a homogeneous complex of Dnmt3L and Dnmt3a

11. Methods  Dnmt3a2, the shorter isoform of Dnmt3a that is the predominant form in embryonic stem cells was selected  For crystallographic reasons, a stable complex of the C-terminal domains from both proteins (Dnmt3a2-C and Dnmt3L-C) that retains substantial methyltransferase activity was focused on

12. Results  The complex is a tetramer: Dnmt3L–Dnmt3a–Dnmt3a– Dnmt3L  Mutagenesis at positions in both interfaces (a-a and a-L) indicate that these interfaces are essential for catalysis  Dnmt3a-Dnmt3a dimerization brings two active sites together  Dimeric Dnmt3a could methylate two CpGs separated by one helical turn in one binding event Dnmt3a Dnmt3l

13. “We observed a highly significant correlation of methylation status at distances of eight to ten base pairs between two CpG sites” Distribution of CpG sites among 12 known maternally imprinted genes, indicated to be Dnmt3a-Dnmt3l targets

14. Protein visualization Visualization tools (working on PC): RasMol / RasTop SwissPDBviewer (sPDBv) Protein Explorer (via the web) And many more…

15. Rastop / Rasmol

16. http://www.geneinfinity.org/rastop/

19. RasTop- main menu פתיחת קובץ סגירת קובץ

20. RasTop - display Wireframe קווים בין אטומים

21. Sphere VDW מנפח כל אטום לפי רדיוס ה- שלוVDW

22. Command editor

29. More on RasTop  RasMol manual RasMol manual RasMol manual  Using RasTop Using RasTop Using RasTop  Commands Commands

30. Structure alignment Essential for: Protein classification Detection of conserved protein folding cores Detection of similarities between domains Detection of similarities in functional binding sites Evolutionary conservation Construction of nonredundant databases

31. Pairwise structure alignment Outline: Given two proteins structures, find the transformation that produces the best superimposition of one protein onto the other

32. Computationally Find the rotations and translations of one of the points set (atoms of protein A) which produce “large” superimpositions on the other points set (atoms of protein B) ? X Y Z X Y Z

33. RMSD Root Mean Square Deviation Average distance between the matched superimposed atoms usually between backbones Cα atoms

34. http://bioinfo3d.cs.tau.ac.il/c_alpha_match  Matches C-alpha atoms  Rigid pairwise alignement  Sequence order independent  Input: two PDB files or PDB IDs with specific chains  Output: a set of high scoring conformations The superimposed structures may be viewed in a PDB viewer The superimposed structures may be viewed in a PDB viewer

35. BOBWHITE QUAIL LYSOZYME HEN EGG WHITE LYSOZYME

36. Results Ranking criteria: 1.Match size 2.RMSD

37. C-alpha correspondence

38. Aligned PDB file

39. http://bioinfo3d.cs.tau.ac.il/FlexProt  Flexible structural alignment  The first structure is assumed to be rigid, while in the second structure potential flexible regions - hinges, are automatically detected  Input: two PDB IDs (specific chain)  Output: list of alignments ranked according to the number of hinges

41. Results

42. Result with 0 hinges:

43. Result with one hinge:

44. http://bioinfo3d.cs.tau.ac.il/MultiProt  Multiple structural alignments of protein structures  Finds the common geometrical cores between the input molecules  Does not require that all the input molecules participate in the alignment  Actually, it efficiently detects high scoring partial multiple alignments for all possible number of molecules from the input  The final structural alignment can either preserve the sequence order (like sequence alignment), or be sequence order independent

46. Results

47. DALI - Distance matrix ALIgnment http://ekhidna.biocenter.helsinki.fi/dali_server/ Concept: “Similar 3D structures have similar inter- residue distances”

48. DALI Algorithm  Generates an inter-residue distance matrix for each protein  The distance matrix contains all pairwise distances (symmetrical)  Dij = distance between C-alpha i and C-alpha j in the same protein  Compares the two distance matrices for a pair of proteins to be aligned

50. DALI Services DALI sever Used by crystallographers to compare a newly solved structure against structures in the PDB DALI database Contains all-vs.-all PDB 3D structure comparisons and thus enables to find structural neighbors of structures that are already in the PDB Pairwise server Pairwise comparison of two structures Dalilite A standalone version of DALI

51. DALI Database http://ekhidna.biocenter.helsinki.fi/dali/start

52. Non-redundant chains - no two chains are more than 90 % sequence identical DALI Database Number of structurally aligned residues Number of residues in the protein Sequence identity of aligned positions

53. Supplementary

54. אלמנטים של מבנה שניוני, קו המחבר בין C-alpha RasTop - Display

55. מחזק מבנה שניוני RasTol - Display

56. Remove rendering Clears the view from previous actions performed

57. לכל חומצה אמינית צבע משלה תצוגה מסוימת בו לכל אטום צבע מסוים עד כמה מיקום האטום קבוע במבנה: אדום- חופש גדול, כחול-קבוע מאוד מבנה שניוני – צהוב- beta sheet, כחול – loops and turns, אדום – alpha helix, לבן – random coil. RasTop - Color

58. RasTop - Labels סימון כל המולקולה או קטעים נבחרים בשם והמספר של חומצת האמינו.

59. The Select Command Select – מגדירה את האזור שעליו נפעל במולקולה - סט האטומים שיעבור מודיפיקציות ע"י שרשרת הפקודות הבאות. הפרמטר של פקודת ה- select הוא “atom expression”. ה - atom expression מגדיר באופן ייחודי קבוצה שרירותית של אטומים בתוך מולקולה. הוא יכול להיות: Primitive expressions, Predefined sets, Within expressions, or logical combination of all above mentioned. In order to display only what we selected, use the command: Edit => restrict

60. The primitive expressions allow to select by:  Atom number - select atomno=102  Residue – select Val52 (select resno=52 or select 52)  Chain id – select :a  List of residue numbers – select 14,92,46  Range of atom numbers – select atomno=>35  A wildcard can be used to specify a whole field: * Any number of characters Atom or residue type – select *.sg (this will select all Sulphur atoms in Cysteine’s side chain) ? Single character wildcard – select ser.c? – will select all carbons in all serine residues.

61. The predefined sets are groups of atoms given the definite names: select helix select hoh (water molecules) select protein There is a list with the predefined sets in the Rasmol reference card (google it)

62. Boolean Expressions And – המשותף לשני תנאים Or – חיבור בין שני תנאים (גם את זה וגם את זה) Not – מה לא לכלול דוגמאות: select tyr and :a → all tyr in ‘a’ chain select tyr or :a → all tyr in the molecule and all ‘a’ chain select not (tyr,:a) → all the molecule beside tyr or ‘a’ chain

63. Using Select Expression

65. …or via the command (edit  command)

66. 1.Spacefill 2.Color picker 3.Make sure you’re on atoms!