2. Vaccine War http://www.pbs.org/wgbh/pages/frontline/vaccines/ “Undergraduate Research Opportunities” will be held Today, Thursday, Jan 13, at 3:30 pm in SL120

3. SEMINARS!!!! The Weiss Laboratory pursues both chemical and biological aspects of chemical biology. Using chemistry to advance a molecular understanding of biology, the lab dissects key events in biology with exceptionally diverse combinatorial libraries as atomic-scale scalpels. Our libraries, collections of different molecules, include virus- displayed proteins and chemically or enzymatically synthesized small molecules. Libraries displayed on the surfaces of viruses also offer essentially universal molecular recognition for chemical sensors, illustrating how biology can advance chemistry. Prof. Gregory Weiss, Department of Chemistry, University of California, Irvine

4. Works exactly like HK. Activated by [AMP] even in the presence of hi [ATP]. Inhibited by hi [ATP] or citrate

5. Mechanism animation

6. Figure 17-8Mechanism for base- catalyzed aldol cleavage. Page 589 Transition state analogs like 2-phosphoglycolate inhibit the enzyme

7. Figure 17-9 Enzymatic mechanism of Class I aldolase. Page 590

8. Enzyme-Substrate Complex trapped by reduction of DHAP with NaBH 4 followed by hydrolysis

9. Figure 16-10 Mechanism of aldose–ketose isomerization. Page 557

20. Figure 17-10Proposed enzymatic mechanism of the TPI reaction: General Acid Catalysis. pKs = 6.5 and 9.5 Like PGI But pK 1 is for GLU! Normal pk? Glu  Asp  activity by 1000! Reaction rate is diffusion limited!! 4.1

21. End of Glycolysis Collection Phase Net result so far? –ATP –NAD + –Carbon

22. Start of energy producing phase of glycolysis: Production of the first hi energy molecule.

23. Figure 17-13a Some reactions employed in elucidating the enzymatic mechanism of GAPDH. (a) The reaction of iodoacetate with an active site Cys residue. (b) Quantitative tritium transfer from substrate to NAD +. Page 596 32 P i also incorporated

24. Animated mechanism

31. Figure 17-14 Enzymatic mechanism of glyceraldehyde-3 phosphate dehydrogenase. Page 596  G o ’ = +6.7 kJ!

33. Figure 17-15Space-filling model of yeast phosphoglycerate kinase showing its deeply clefted bilobal structure. Page 597

34. Figure 17-16Mechanism of the PGK reaction. Page 597  G o ’ = -49.4 kJ!  G o ’ = -12.1 kJ

36. Mutases move functional groups: 3PG  2PG Animated mechanism

37. Figure 17-19The pathway for the synthesis and degradation of 2,3-BPG in erythrocytes is a detour from the glycolytic pathway. Page 600

38. Figure 17-20The oxygen-saturation curves of hemoglobin (red) in normal erythrocytes and those from patients with hexokinase (green) and pyruvate kinase deficiencies (purple). Page 600  BPG  BPG

42. Figure 17- 18 Prop osed reaction mechanis m for phospho- glycerate mutase. Page 599 Phosphorylated active site Bisphospho- intermediate.

45. Figure 17-22Mechanism of the reaction catalyzed by pyruvate kinase.

47. Let's sing!! http://www.csulb.edu/~cohlberg/songbook. html

48. Lyrics http://books.google.com/books?id=oq9ENyL_d9YC&lpg=PP1&pg=PA1#v=on epage&q&f=false

49. Figure 17-21 Proposed reaction mechanism of enolase. Page 601 F - binds P i + Mg +2 Potent inhibitor

50. Figure 17-23The active site region of porcine H 4 LDH in complex with S-lac-NAD +, a covalent adduct of lactate and NAD +. Page 603

51. Figure 17-24 Reaction mechanism of lactate dehydrogena se. Page 603

52. Figure 17-25The two reactions of alcoholic fermentation. Page 604

53. Figure 17-26Thiamine pyrophosphate. Page 604

54. Figure 17-27 Reacti on mechanism of pyruvate decarboxyla se. Page 605

55. Figure 17-29The formation of the active ylid form of TPP in the pyruvate decarboxylase reaction. Page 606

56. Figure 17-30 The reaction mechanism of alcohol dehydrogenase involves direct hydride transfer of the pro-R hydrogen of NADH to the re face of acetaldehyde. Page 606

57. Table 17-2Some Effectors of the Nonequilibrium Enzymes of Glycolysis. Page 613

58. Figure 17-32aX-Ray structure of PFK. (a) A ribbon diagram showing two subunits of the tetrameric E. coli protein. Page 614 Mg +2 ATP F6P

59. Figure 17-33PFK activity versus F6P concentration. Page 615

60. Figure 17-35Metabolism of fructose. Page 619

61. Figure 17-36Metabolism of galactose. Page 621

62. Figure 17-37Metabolism of mannose. Page 621

63. Figure 17-31Schematic diagram of the plasmid constructed to control the amount of citrate synthase produced by E. coli. Page 609

64. “Alfonse, Biochemistry makes my head hurt!!” \