1. Biosynthesis of 生科系博士班 884234 李曉青 資工系博士班 914338 張君天 資工系博士班 928307 林沿妊 Group 3

2. Powerful antioxidant, reduces the risk of –Coronary heart disease by preventing oxidation of low density lipoprotein (LDL) cholesterol –Atherosclerosis by protecting plasma lipids from oxidation –Oxidative damage of lymphocyte DNA –Cancer

5. Protein ID Protein nameGene 2.2.1.71-deoxy-D-xylulose-5-phosphate synthase dxs E.coli 1.1.1267 2-C-methyl-D-erythritol 4-phosphate (MEP) synthasedxr E.coli 2.7.7.60 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase ygbP E.coli 2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ychB E.coli 4.6.1.12 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseygbB E.coli 1.17.4.3 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthasegcpE E.coli 1.17.1.24-hydroxy-3-methylbut-2-enyl diphosphate reductaselytB E.coli 5.3.3.2 isopentenyl-diphosphate delta-isomerase b2889E.coli 2.5.1.10 farnesyl-diphosphate synthaseispA E.coli 2.5.1.29 geranylgeranyl pyrophosphate synthetasecrtE E.uredovora 2.5.1.32 phytoene synthetasecrtB E.uredovora 1.3.99-oxidoreductase crtI E.uredovora 1.14.99.3 0 zeta-carotene desaturasecrtQ E.uredovora

6. dxs:DXP synthase idi: IPP isomerase ispA: Farnesyl-PP synthase

7. crt E: GGPP synthase crt B: phytoene synthase crt I: lycopene synthase from E.uredovora gps:ggpp synthase from A.fulgidus

8. Red : E.coli Blue : A.fulgidus Green: E.uredovora

9. Wang CW, Oh MK, Liao JC. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol Bioeng. 1999 Jan 20;62(2):235-41. Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol. 1990 Dec;172(12):6704-12. Farmer WR, Liao JC. Improving lycopene production in Escherichia coli by engineering metabolic control. Nat Biotechnol. 2000 May;18(5):533-7. References