1. Photodynamic perforation of cell membrane on micro channel array toward intracellular technology Advisor ： Cheng-Hsien Liu Reporter ： Yu-Sheng Lin Date ： 2007.06.20 K. Iso 1, T. K. Saito 2, H. Muguruma 3, H. Tabata 2, S. Konishi 1 1 Ritsumeikan University, 2 Osaka University 3 Shibaura Institute of Technology, Japan MEMS 2007 pp.445~448

2. 2 Outline Introduction Operational procedure Design and fabrication Experiment and results Conclusion

3. 3 Ref: Wikipedia Patch-clamp Introduction Cell Figure 1. Schematic drawing of whole-cell patch-clamp Ref ： T.K. Saito, 2002

4. 4 Introduction (cont.) Ref ： T.K. Saito, 2002

5. 5 Operational procedure Medium filling into the device Clamping the cell Exchange to the PS incl. medium Excite PS with UV to perforate membrane

6. 6 Fabrication SOI

7. 7 Fabrication results Upper structure (Top view) Upper structure (Back view) Lower structure Implemented device (Top view)

8. 8 Experiment setup

9. 9 Results Micro channel in focusCell in focus Before BAT excitationAfter BAT excitation

10. 10 Results (cont.)

11. 11 Conclusion Photodynamic perforation of cell membrane performed successfully. Use MEMS technology to improve the patch-clamp technique. The perforation method is expected to improve intracellular technologies.

12. 12 Thank you for your attention !