1. Fish Heath Management Lab 5: Histology (microanatomy) Tarah Johnson March 2 nd, 2009

2. Objectives Be able to microscopically identify cells and structures in the liver, kidney, and gills Have the opportunity to microscopically examine histological sections of other fish organs (eyes, head, muscle, etc.)

3. Liver Hepatocytes Hepatic Sinusoid Central Vein and or Hepatic Portal Vein Bile Duct Arteriole Glycogen Vacuole

4. Central Vein and Hepatic Sinusoids Erythrocytes visible within the central vein Hepatic sinusoids 400x

5. Bile DuctArteriole A. Glycogen Vacuole A 400x Erythrocytes visible within arteriole 400x

6. Head Kidney Interrenal gland Chromaffin cell Postcardinal vein Hematopoietic tissue

7. A B C A.Interrenal Gland B.Chromaffin Cell C.Postcardinal vein 400x

8. A. Postcardinal vein B. Hematopoietic tissue 40x A B

9. Trunk Kidney Nephron (may not observe a complete nephron): Renal corpuscle, proximal tube, distal tubule, and collecting duct Renal Corpuscle: Glomerulus, Bowman’s capsule, basement membrane of glomerulus Melanocytes Collecting duct and/or Mesonephric duct Connective tissue

10. A B C A.Renal Corpuscle B.Proximal Tubule C.Distal Tubule 400x

11. A.Glomerulus B.Basement membrane of glomerulus C.Bowman’s capsule A C B

12. A B A.Mesonephric Duct B.Melanocytes C.Connective tissue 400x C

13. Gills Filament Lamella Goblet (or mucous) cell Chloride cell Pillar (or pilaster) cell Gill filament cartilage

14. Gill filaments and lamellae Lamella A A.Filament cartilage B.Pillar cell C.Chloride cell B 40x 400x C

15. Mucous or Goblet Cells 400x

16. Laboratory Steps Use proper microscope handling procedures (distributed in Lab 2) Examine histology slides of liver, kidney, and gills Examine additional histology slides of other organs

17. Be Sure to… Clean oil immersion lenses Clean slides and return to proper case (the same one you took it from) Turn off microscopes and leave on low power objective when done