1. We have viruses!! Tucson High School Biotechnology Course Spring 2010

2. Results from Last Class Synechococcus Prochlorococcus

3. Results from Last Class: Controls Negative Control Positive Control 10 4 dilution (higher concentration of viruses) Positive Control 10 6 dilution (lower concentration of viruses

4. Results from Last Class: Tara Oceans Samples No Plaques: 11 Tara Samples Plaques!! 6 Tara Samples

5. Results from Tara Oceans

6. Now What?? Pick Plaques! Yes! No! 3 μl

7. What’s Next? Generate many more viruses We need a lot! Each virus: ~55 ag DNA (0.000000000000000055 g) We need: a few μg (0.000001 g) => 1.8 x 10 10 viruses (18000000000 viruses)

8. A reminder Overlays are NOT solid! **Do NOT tilt, shake, jostle, or turn over your plates!**