1. Week 6: Cell Morphology Wright stain RBC morphology Anisocytosis
Poikilocytosis
WBC morphology
WBC differentials
Artifacts

2. Wright Stain Romanowsky stain family Polychromatic
Absolute methanol fixative
Methylene blue - basophilic components
Eosin - acidophilic components

3. Smear Making
Essential in morphology
Wedge method
Thickness and length

7. Key Features Size Nuclear shape Chromatin features
Granules and other inclusions
Color

8. Leukocytes Granulocytes: have specific granules Non-granulocytes
Eosinophil
Basophil
Neutrophil
Non-granulocytes
Lymphocyte
Monocyte

9. Granulocytes Eosinophil Large orange granules <2% in circulation
Usually bilobed
Increased in allergy, parasites

10. Granulocytes Basophil Large black-purple granules
<1% in circulation
Increased in allergy, CML

11. Granulocytes Segmented Neutrophil Band Small purple granules
54-62% in circulation
Coarse chromatin
Increased in bacterial infection
Band
No nuclear filament
3-5% in circulation

12. Non-Granulocytes Lymphocyte Relatively small Round or oval nucleus
Smudged chromatin
Basophilic cytoplasm
Increased in viral infection
% (more in children)

13. Non-Granulocytes Monocyte Relatively large
Grey cytoplasm with vacuoles
Irregular nucleus
Linear chromatin
Increased in chronic infection
3 - 7%

14. Platelets Cytoplasmic fragments of large megakaryocyte
2-3 mm in diameter with granules
Promotes clot formation
8-20 per oil immersion field
150, ,000/mL

15. Erythrocyte Morphology
Normal: biconcave disc of 7.5 mm diameter
Has central pallor (1/3 of diameter) on smear
No inclusions

17. Good Area to Evaluate Morphology

18. Anisocytosis: Variation in Size
Microcytes
Macrocytes

19. Poikilocytosis: Abnormal Shape
Target Cells
Hypochromic

20. Poikilocytosis
Schistocyte
Sickle Cell

21. Poikilocytosis
Teardrop Cell
Spherocyte

22. Poikilocytosis
Elliptocyte
Stomatocyte

23. RBC Inclusions, etc.
Polychromasia
Howell-Jolly Bodies

24. RBC Inclusions
Cabot Ring
Basophilic Stippling

25. RBC Inclusions, etc.
Malaria
Rouleaux

26. Too Thick
Too Alkaline
Too Thin
Too Acidic

27. Unacceptable Slides