1. LUCIFERASE A. Cengiz Öztireli

2. BIOLUMINESCENCE BIOLUMINESCENCE (LIVING LIGHT) Milky Sea

3. BIOLUMINESCENCE Applications

4. BIOLUMINESCENCE Sea AnemonesBacteriaDinoflagellates

5. BIOLUMINESCENCE FIREFLYES (PHOTINUS) Attracting mates Attracting prey

6. LUCIFERASE FIREFLY BACTERIA

7. BIOLUMINESCENCE 8 ATP → 1 Green Photon Reaction: Luciferin Luciferase + O 2 Inter.P*P* P + hv ATP

8. BIOLUMINESCENCE Color  P *  P * + F → F * + P  Proteins around  Structure

9. PHOTINUS PYRALIS (FIREFLY)

10. PHOTINUS PYRALIS LUCIFERASE Luciferase + Luc-COOH ATP Luciferase: Luc-CO-AMP O2O2 Luciferase: Luc=O *

11. PHOTINUS PYRALIS LUCIFERASE N-Terminal C-Terminal Cleft Structure and Functioning

12. CONCLUSIONS THANK YOU