1. Uses of the CCD n Image fluorescence - living or fixed, multiple colors n Image phase or DIC - living or fixed

2. Elements of the CCD workstation (located in Rm 012) n Microscope n CCD camera n Computers for image aquisition/analysis n Filter wheels n Acquisition/analysis software- Openlab n CO2 incubator and heating stage n Focus drive

3. Elements of the CCD workstation Leica DMIRB - inverted microscope n 20X phase - adjustable focal length - to be used with anything from thick petri plastic to thin (0.17um) coverslips n 40X phase- adjustable focal length - n 63X phase- adjustable focal length - n 63X water immersion (no phase) n Oil immersion objectives can be shared with the confocal

4. Elements of the CCD workstation CCD camera n Princeton Instruments MicroMax n 12-16 bit, 1000X800 pixels n Back illuminated, cooled CCD n High resolution/high sensitivity

5. Elements of the CCD workstation Computers for image acquisition and analysis n Acquisition computer (Mac G3, 256 MB RAM, 4GB) is located in room with camera - data should be transferred to CD or the analysis computer soon after acquisition because files are large. n Analysis computer is located in Room 123

6. Elements of the CCD workstation Filter Wheels n Emission/Excitation Filter Wheels n Filters Include: n FITC n CY3/Texas Red n GFP n BFP

7. Elements of the CCD workstation Microscope-attached CO2 incubator and heating stage n Allows long-term experiments at constant temperature and % CO2

8. Elements of the CCD workstation Acquisition and Analysis Software - Openlab n Macros control camera/focus drive/filter wheels for long-term acquisition n Analysis package allows manipulation of images for presentation and quantitation

9. Elements of the CCD workstation Acquisition and Analysis Software - Openlab

10. Elements of the CCD workstation Focus Drive n Allows acquisition from multiple focal planes - can compensate for movement of living structures n Can be used to obtain confocal images in conjunction with the Silicon Graphics program Imaris

11. Elements of the CCD workstation Focus Drive