1. 29 September and 1 October, 2004 Chapter 11 Transposition and Site- Specific Recombination

2. Overview Conservative Site-Specific Recombination (CSSR) may involve insertion, deletion, or inversion of DNA sequences. Site-specific recombinases have a mechanism that includes a DNA- protein covalent intermediate. CSSR may be regulated by the presence or absence of accessory proteins. Resolvases are CSSR recombinases that disentangle circular chromosomes. Transposons move using recombination pathways. Transposons may be autonomous or nonautonomous. DNA transposons and viral-like retrotransposons move via a cut/paste mechanism. Retrotransposon movement involves reverse transcription. Some transposons regulate copy number or control target site selection through the use of proteins or antisense RNA. V(D)J recombination uses regulated, specific recombination to generate immune diversity.

3. Recombination and Transposition

4. CSSR: Prophage Insertion

5. Three Classes of CSSR

6. Recombinase Recognition Sites

7. Recombinase Mechanism

8. Serine Recombinases

9. Tyrosine Recombinases

10. Mechanism of Cre Recombinase

11. Cre-DNA Structure

12. Lambda integration requires architectural proteins.

13. Int and IHF stabilize bent DNA.

14. Hin Inversion

15. Hin inversion requires Fis bound at an enhancer.

16. Resolvases disentangle circular DNAs after replication.

17. FtsK Regulation of the Xer Resolvase Mechanism

18. FtsK is present at the division closure site.

19. Conservative and Replicative Transposition

20. Transposons in Several Genomes

21. Types of Transposons

22. Cut and Paste Transposition

23. Three Mechanisms for Cleaving the Nontransferred Strand

24. Replicative Transposition

25. Retrotransposon Movement

28. DNA Transposases and retroviral integrases are members of the same protein superfamily.

29. LINE poly-A Retrotransposon Movement

30. Tn10 achieves antisense copy control by overlapping promoters.

31. Antisense Copy Control

32. Tn10 transposase promoter is active only when hemimethylated.

33. MuA and MuB participate in selecting Mu transposition target sequences.

34. MuA disrupts MuB assembly, conferring transposition target immunity.

35. Clustered Integration of Yeast Ty Elements

36. Antibody

37. V(D)J Recombination

38. Recombination Signal Sequences

39. V(D)J Recombination Mechanism

41. Title

42. LINES and SINES Again?