1. Asymmetric division Text and image sources are included using the notes function of this file

2. All centrosomes are not equivalent! DNA Microtubules IoDPP mRNA

3. Yeast spindle orientation GFP-tubulin Dyn1:His3 Video

4. MTOC reorientation to the immunological synapse in single positive mature T-cells

5. Ash1 mRNA (encoding a transcriptional repressor of MAT switching) is transported into the daughter in budding yeast Locasome transport requires formin-initiated microfilament cables and a myosin V family member (Myo4p)

6. 3’UTR structure is important Ash1 mRNA ash1 mutants don’t switch

7. Proteins are segregated to the bud too Both the Ist2p protein and mRNA are localized to the bud

8. Caenorhabditis elegans Video

9. Caenorhabditis elegans development GFP::histone fusion Female Male Pronuclei Video

10. P-granule partitioning

11. P-granules fill at the NE

12. Cytochalasin Wild-type partitioning Nocodazole GFP::P granules at the movies 3 Videos

13. Localization of Par-2::GFP Video

14. Par2::GFP is increasingly restricted to the P lineage Video

15. Par mutant phenotypes

16. Setting it all up Mex-5 Mex-6 Par3/6/aPKC Pie-1 Par-1 Par-2 P-granules

17. Pop goes the weasel!

18. Xenopus development

19. The first cleavages split the embryo on its three axes and continue to generate a mass of cells Video

20. Veg1 binds to VERA on ER Gel shift assayDensity gradient centrifugation

21. METRO organizes traffic

22. Localization of mRNAs in Drosophila oskarbicoid

23. Drosophila disconnects mitosis from cytokinesis GFP-tau marks microtubules Video

24. Pole cells get polar granules

25. Microinjection rescues the day!

26. Anterior-Posterior Gradients

27. Cascades of gene activity set up the Drosophila body plan

28. Loss of Bicoid results in no head! Wt larval head bicoid null

29. Mislocalization of Nanos gives two tails

30. Cellularization traps nuclei with different RNA populations that are translated to make create different microenvironments Bicoid, even-skipped and caudal

31. Partitioning of Cdx2 in the mouse embryo Deb K, Sivaguru M, Yong HY, Roberts RM. Cdx2 gene expression and trophectoderm lineage specification in mouse embryos. Science. 2006 Feb 17;311(5763):992-6.

32. Another Drosophila example: neurogenesis Muscle precursors Postmitotic Neurons Sensory precursors Apical surface Notch GMC

33. Cell division in Drosophila epidermoblasts Basal Apical Video

34. Neuroblast spindle rotation in metaphase Basal Apical Video

35. Asymmetric neuroblast division Basal Apical Video

36. Numb partitioning DNA and Numb protein and centrosomes Anaphase Telophase Neuroblast GMC Apical Basal

37. GFP-PON (partner of Numb) basal apical Video

38. PON localization precedes spindle rotation Cell expressing both GFP::PON and GFP::tubulin Video

39. Asymmetric components Inscuteable + Partner of Inscuteable (PINS) Baz(Par3)/Par6/aPKC and Galphai (G-protein) Numb Partner of Numb (PON) Neuroblast (Apical) (Basal) GMC MyoVI Miranda Stauffen (RNA binding) + Prospero ( homeodomain transcr. factor) +-+-

40. Inscuteable RNAi disrupts rotation Note cell still appears to divide asymmetrically Basal Apical Video

41. Mutations in the tumor suppressor Discs Large cause loss of polarioty Wild type Mutant basal apical