1. 研習報告與討論研習報告與討論 蔡騰寬 901607 中研院生醫所林陽生老師實驗室

2. Interaction of TCF4 with DP103 and FHL3

3. Abstract Wnt signal pathway, especially beta- catenin/TCF4 complex, plays a critical role in colorectal cancer development. By means of yeast two-hybrid assay and ECL Western blotting, DP103 and FHL3, respectively show interaction with TCF4.

4. Introduction Wnt signaling plays a crucial role in the etiology of colorectal cancer. When Wnt signal is absent, the levels of cytoplasmic beta-catenin are low, and T cell factor 4 (TCF4) in the nucleus is repressed.

5. Canonical Wnt Pathway

6. Materials and Methods TCF4 Cloning Yeast Two-Hybrid Assay DNA Sequencing ECL Western Blotting

7. TCF4 Cloning

8. Yeast Two-Hybrid Assay

9. Reporter constructs

10. Yeast Two-Hybrid Assay Yeast Colonies

11. ECL Western Blotting

12. Results Transformed E. coli Is Expressible Interaction of TCF4 Is Observable in Yeast Interaction of TCF4 Is Detectable in Animal Cell

13. Discussion Benefits in Yeast Two-Hybrid System Limitations in Yeast Two-Hybrid System Proposed Model for TCF4, DP103 and FHL3 Expression

15. Conclusion Interaction of TCF4 with DP103 and FHL3 is detectable in yeast and animal cell.