1. Accurate Day 1 blood testing for SARS Securing a better future SARS 血漿 RNA 測試 保障美好明天

2. SARS detection by current tests 現有的 SARS 病毒測試方法

3. Anti-virus antibody testing in blood 病毒抗體測試 Indirect testing 間接 Positive after 14 days 14 日後才有用

4. Virus culture 病毒種植 Takes several days 需時數天

5. PolS EMN

6. Nasopharyngeal aspirate 鼻咽液 32% day 3 68% day 14 Faeces 糞便 97% day 14 Urine 尿液 42% day 15

7. New SARS blood test 新的 SARS 病毒血 液 測試

8. Why a blood test? 為何用血液 ?

9. PolS EMN SARS Plasma RNA test SARS 血漿 RNA 測試

12. Day 1 of hospital admission 入院後的首天 Detects 80% of all patients with SARS 準確識別 80% SARS 病人 Detects 100% of patients who will need ICU admission 準確預測需要深切治療的病人 No signal for non-SARS patients 沒有錯認非 SARS 病人

13. Quantitative Dimension 量化平核

14. ICU and non-ICU patients 深切治療 及 非深切治療 28 times increase

15. Outcome comparison 死亡個案比較 5 times increase

16. Serial Analysis 連續觀察

17. 1 2 3 4 5 1 10 100 1000 10000 100000 SARS CoV (copies/mL) Day non-ICU patient ICU patient

18. Future Applications 未來的應用 Guidance to drug treatment 藥物治療跟進 Testing to new drugs 新藥試驗 Virus load and infectivity 衡量病毒數量及傳播性

19. Conclusions 總結 Quantitative blood test 量化測試 Day 1 testing detects 80% of cases 準確 Day 1 testing reveals severity 預測嚴重性 Continuous monitoring 連續觀察 Powerful research tool 重要研究工具

20. Thank you