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09 生醫奈米粒子 同材料奈米尺寸的粒子與較大尺寸 的粒子,其光學等物理性質相異. Gold nanoparticles The Lycurgus Cup dates from Roman times. The glass appears green in daylight (reflected light),

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Presentation on theme: "09 生醫奈米粒子 同材料奈米尺寸的粒子與較大尺寸 的粒子,其光學等物理性質相異. Gold nanoparticles The Lycurgus Cup dates from Roman times. The glass appears green in daylight (reflected light),"— Presentation transcript:

1 09 生醫奈米粒子 同材料奈米尺寸的粒子與較大尺寸 的粒子,其光學等物理性質相異

2 Gold nanoparticles The Lycurgus Cup dates from Roman times. The glass appears green in daylight (reflected light), but red when light is transmitted from the inside of the vessel.

3 their easily controllable-size distribution long-term stability and friendly biocompatibility with antibodies, antigen proteins, DNA and RNA Gold nanoparticles in assay

4 現行之 DNA 檢測技術中,以 PCR 為首之 檢測技術,以及螢光染色法與放射性標 誌等檢測方法。目前這些方法所能夠偵 測之最小值有限,而且敏感度更是受到 許多些微因素的影響。 [G. H. Keller, M. M. Manak and N.Y. Stockton, “DNA Probes” (1989). ] ssDNA-Au nanoparticles

5 1. Thaxton, C.S., Georganopoulou, D.G., Mirkin, C.A. 2006 Clin. Chim. Acta, 363, 120-126. 2. Sato, K., Hosokawa, K., Maeda, M. 2003 JACS, 125, 8102-8103.

6 DNA 結合金奈米粒子之分析 (a) (b) 圖 12 以 TEM 觀測 DNA- 金奈米粒子分佈情形 (a) DNA(SH 1,SH2)/ 金奈米 粒子 (b) 加入 Linker 1 後呈現聚集 (assembly) 的狀態 穿遂式電子顯微鏡 (TEM) 之分析

7 Single-Nucleotide Polymorphism Jishan Li, Xia Chu, Yali Liu, Jian-Hui Jiang, Zhimin He, Zhiwei Zhang, Guoli Shen and Ru-Qin Yu Nucleic Acids Research, 2005, Vol. 33, No. 19 e168

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11 Antibody-Au nanoparticles 金奈米粒子的應用 : 驗孕片之原理 ( 免疫層析法 )

12 Li, Alastair W. Wark, Hye Jin Lee, and Robert M. Corn Anal. Chem.2006, 78,3158-3164

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14 Silver Enhancement

15 Colony Printing Salmonella 綠膿桿菌

16 複合奈米粒子 : 有趣的人造原子 陳東煌 成功大學化工系

17 複合奈米粒子

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19 (a) (b)

20 Negative control 100 fmol 10 fmol 1 fmol 100 amol 10 amol 1 amol 100 zmol 10 zmol 1 zmol (A) (B)


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