1. MARS: Microarray analysis, retrieval, and storage system Albert F. Cervantes

2. Overview Microarray Background Mars Implementation  Software Architecture  Systems  Data Interfaces  Web Services  Access Control Remarks

3. Microarray Background Popular technique  Study gene expression patterns on genomic scale Produces wealth of data  Tens of thousands of elements  Annotated by dozens of parameters. Need for a fast, robust cataloging method Solution: Mars  MIAME software suite Minimum Information about a Microarray Experiment Microarray Gene Expression Data society initiative

4. Software Architecture J2EE platform 3 tier architecture  Enterprise Information System Data in relational database  Application Manage DB interaction & data access  Presentation Web-server

5. Software Architecture Maurer, et al. MARS: Microarray analysis, retrieval, and storage system. BMC Bioinformatics 2005, 6:101

6. Systems – 5 major sub-groups DB schema, Business Model, & Web Interface  Microarray production LIMS – laboratory information management sys  Sample preparation  Hybridization and raw data management  Experiment annotation  Quality Management

7. Data Interfaces MAGE-ML  MicroArray Gene Expression Markup Language  Initiative by MGED Microarray Gene Expression Data Society Import Interface  Define file parsers  Use generic files  Tab-delimited files Allows for storage of previous data

8. Web Services MARSExplorer  Java library distribution Software developers  Data access via SOAP Simple Object Access Protocol

9. Access Control AAS  Authentication and authorization system Fine grained user access levels Prevention  Unauthorized access  Data modification Legacy code integration

10. Access Control Maurer, et al. MARS: Microarray analysis, retrieval, and storage system. BMC Bioinformatics 2005, 6:101

11. Remarks Novel solution  Catalog & archive experimental data in microarray production Obsolescence Resistant  Awareness of data annotation standards  Scalable and distributable Offers a complete laboratory solution