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Project 1 Frog Gels: Analysis of Skin John Donich Claudia Morales Kevin Berry
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Project description §Goal: to describe the set of proteins in 7.5% gels and 12 % gels… §Are males differentiable from females…? §Are individuals differentiable?
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An ideal model… §There is little variation between frogs of the same sex §There’s a distinct diference in the set between sexes §A protein that shows in only one of the sexes
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Method §Measured relative mobility of protien bands §Plotted standards’ relative mobility vs. Log Molecular Weight §Chose a model and fit the data §Looked for patterns within individuals and between sexes
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Choosing models §Linear Model for 7.5% gels… l Only 3 Degrees of Freedom (5 Standards) l Linear Model had smallest confidence interval §Cubic Model for 12% gels… l 5 Degrees of Freedom (7 Standards) l Cubic and Linear models had similar confidence intervals, but R 2 -adj better for cubic (99% vs 96%)
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Conclusions § The variation among individuals is present §The sex of a frog cannot be determined by protein gel analysis §There is no single protein that distinguishes sexes
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