1. Instrument Performance --Tests Field illumination (plastic slides, beads) Axial resolution (mirror) Chromatic aberration (1 u beads, mirror) Laser power (meter, test sample) Laser stability (plastic slide, transmission detector) Beam alignment (focal check,mirror,bead)

4. Deviation + 8%

5. Deviation + 4%

7. Deviation + 17%

8. 647 568 488 Deviation = +10%, +17%, +13%

9. UV Field Illumination 100X Objective (1.4 NA) 12.5% intervals INTENSITY=230 123 47

10. 5X Objective (.25 NA) 12.5% intervals

11. 20X Objective (.7 NA) 12.5% intervals

12. Axial Registration Mirror Test 63X objective (1.2NA) (Chromatic Aberration)

13. 63x ( 1.2NA) objective 647 488 568

14. 647568 488

15. Bad Galvanometer

16. 568 nm 488 nm + 568nm 568 nm

17. Test Samples Beads: Spherotech 10 micron, MP focal check, 1 micron, submicron Front silvered mirror Silicon chip Biological samples-Diatoms, Pollen, MP Fluo slide, User defined specimen Fluorescence Plastic Slide

18. ABC D E F C

19. Lower NA’s --Less resolution Longer working distance Cleaner working environment Dry Objectives