1. Molecular Microbiology

2. Genetic Recombination Vertical –Binary fission –Budding Horizontal –Conjugation –Transduction –Transformation

3. Conjugation

4. Transduction

5. Transformation

6. Griffith Experiment

7. Biotechnology Recombinant DNA Genetic Engineering Disease treatments –SCID –SSA –CF Make products –Vaccines –Enzymes –Insulin –Hormones

8. Plasmids DNA Replicate independently Genetic Vectors –Small –Stable –Marker inserted –Gene expression

9. Background Donor Recipient Competent Cell –Natural Change cell wall Change PM –Artificial Temperature Salts (CaCl2) Marker Cloning Site

10. LB Broth and Agar Lysogeny Broth –Luria Broth –Luria-Bertani Nutritionally Rich –Peptides, casein –Vitamins –Minerals (NaCl) –Trace elements Biotech –Cultivate E.coli

11. Procedure Follow lab manual!! Reader –Directs –Keeps time Materials –LB plates 2 types –LB –LB-amp 2 situations –Before –After Label, Label, Label –pGreen plasmid –CaCl2 –Micropipets –Spreader –Loop inoculation Temperature –Incubator –Baths Ice Heat

12. Results Marker –Fluorescence Cloning site –R-ampicillin Colony observation –Natural light –UV light Calculate transformation efficiency

13. Questions? Glow Fish