2. FROM IMAGES TO ANSWERS Image Pro Plus 5.1 Introduction

3. Image-Pro Plus 5.1 More Answers from your Images!

4. Image Pro Plus 5.1 New Features Improved Memory Manager –Load Image Sets Larger than Available RAM –Breaks Through the “Hard Wall” at 2GB for Windows ® Applications* with Image Stacks –Useful with Today’s Larger Digital Image Stacks –Works Seamlessly and Silently in the Background * Cannot open a single image larger than 2GB

5. Image Pro Plus 5.1 New Features Macro Player –Load and run the various macros within IPP –Demonstrating a range of applications

6. Image Pro Plus 5.1 New Features Stitching and Tiling –Align and stitch images together –Save or load a set of calculated alignments to be used on Fluorescence images

7. Image Pro Plus 5.1 New Features Alignment correction –align individual images, or images in a sequence, for translation, rotation, or scaling –Useful for images taken over time, images with some camera movement, aligning channels in multiple images from a beam splitter, or correcting for offset angles through a stereo microscope Z-stack.

8. Image Pro Plus 5.1 New Features Improved Automatic Tracking –Correlation Tracking on Active AOI –Full Correlation or Phase Correlation Options for Tracking under Different Image Conditions –More than 50 Morphological Measurements available including Intensity Tracking –Smart Search Radius Detects Object Movement from Image –New Coherence Filter Adjusts for Neighbor Vectors –1-Button Static or Dynamic Intensity Tracking

9. Image Pro Plus 5.1 New Features Toolbar Extensions Use Existing Menu Icons Create New Icons Include Self-Contained Macros with no External Parameters

10. IQbase (Now Included with Image Pro Plus) Powering Image Informatics Manages Images and Data Drives Discovery Facilitates Collaboration Easy to Use Works the Way You Do Grows with Your Organization (Scalable)

11. Gradient painting macro Generate Test strips Create Surface plot VRML (Virtual Reality Modeling Language ) VRML Viewing plug-in required (http://www.parallelgraphics.com/products/cortona/download /) MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

14. FRET Calculations MACROS in IPP (FRET)

15. Image-Pro Family Software Series Perfect for Basic Imaging or Capture Stations More Advanced than Image- Pro Express, Includes Support for Plug-Ins The Ultimate Imaging Package, Includes Support for Macros and Plug-Ins

16. Image-Pro Family Bundles Image-Pro Advanced Microscopy Suite This complete solution for high-level microscopy analysis and acquisition combines Image-Pro Plus 5.1 with powerful tools for multi-dimensional microscope control, image deconvolution, and 3D rendering.Image-Pro Plus 5.1 Image-Pro Multi-Dimensional Acquisition Ideal for automated microscopy work, this package combines Image-Pro Plus 5.1 with tools for multi- dimensional microscope control and acquisition.Image-Pro Plus 5.1 Image-Pro 3D Suite Ideal for deconvolution and interactive control and analysis of volume stacks, this package combines Image-Pro Plus 5.1 with deconvolution and rendering tools. Image-Pro Plus 5.1 Image-Pro Microscope Control The Image-Pro Microscope Control (MC) package combines Image-Pro Plus Version 5.1 with tools to control and program all major automated microscopes.Image-Pro Plus Version 5.1

17. Image-Pro Family Bundles Image-Pro 3D Discovery Perfect for confocal microscopy, the Image-Pro 3D Discovery package combines Image-Pro Discovery 5.1 with tools to provide full interaction and analysis of all 3D volumes. It includes major confocal microscope support. Image-Pro Analyzer Ideal for networked users who want advanced analysis tools but do not need image capture functionality at each work station. Image-Pro Analyzer offers all of the powerful analysis features of Image-Pro Plus 5.1, but does not include support for acquisition.Image-Pro Plus 5.1 10 Unit Network 5 Unit Network

18. Image-Pro Advanced Microscopy Suite Image-Pro 5.1 Advanced Microscopy Suite Includes: Image Pro Plus 5.1 3D Constructor Sharpstack (SharpStack Plus available as an Option) AFA Scope-Pro IQbase AMS Benefits Lower Customer Prices Avoids Version Conflicts One CD Loads All Programs Upgradeable from Earlier Image Pro Versions

19. Image-Pro Family Bundles Image-Pro Multi-Dimensional Acquisition Includes: Image Pro Plus v. 5.1 Advanced Fluorescence Acquisition 5.0 Scope-Pro 5.0 IQbase MDA Benefits Lower Customer Prices Avoids Version Conflicts One CD Loads All Programs Upgradeable from Earlier Image Pro Versions

20. Image-Pro Family Bundles Image-Pro Microscope Control Includes: Image Pro Plus 5.1 Scope-Pro 5.0 IQbase MC Benefits Lower Customer Prices Avoids Version Conflicts One CD Loads All Programs Upgradeable from Earlier Image Pro Versions

21. Image-Pro Family Bundles Image-Pro 3D Suite Includes: Image Pro Plus v. 5.1 Advanced Fluorescence Acquisition 5.0 Scope-Pro 5.0 IQbase MDA Benefits Lower Customer Prices Avoids Version Conflicts One CD Loads All Programs Upgradeable from Earlier Image Pro Versions

22. Image-Pro Family Bundles Image-Pro 3D Discovery Includes: Image Pro Discovery 5.1 3D Constructor 5.0 IQbase MC Benefits Lower Customer Prices Upgradeable from Earlier Image Pro Versions Avoids Version Conflicts One CD Loads All Programs Standalone Version Ideal for Confocal Applications

23. Image-Pro Family Bundles Image-Pro Analyzer Includes: Image Pro Plus v. 5.1 Network Analysis Only (No Capture) IQbase Analyzer Benefits Lower Customer Prices (Network 5 or 10 Seats)

24. Image Pro Multi-Dimensional Acquisition

26. Image Pro Microscope Control Software control of stages, filter wheels, shutters, z-motors, microscopes Ludl, Marzhauzer, Prior, ASI and Objective Imaging Leica, Olympus, Zeiss, Nikon Sutter, Physik

27. Image Pro 3D Suite 3D reconstruction, visualization and volumetric and spatial measurements Major Features –Free Rotation of Image Stacks –Extensive Animation & Display Tools –Orthogonal & Oblique Sectioning –Numerous Spatial & Volumetric Measurements –Time Resolved Tracking of Objects

28. Image Pro 3D Suite Spatial Measurements

29. Image Pro 3D Suite Automatic Volumetric Measurements

30. Image Pro 3D Suite Automatic Volumetric Measurements Over Time 4D Tracking of objects!!

31. Image Pro 3D Suite Neuron Analyzing (Neuron Spines) !!

32. Image Pro 3D Suite 3D Suite Includes: No Neighbor, Nearest Neighbor Haze Removal and Inverse Filter Haze Restoration Algorithms These Algorithms are not Appropriate for Confocal Images An Option is Available which Includes 2D Blind and 3D Blind Deconvolution Algorithms

33. Image-Pro Family Modules Automate Motorized Microscopes or Devices (available separately until 12/2004) Color-Pro Color Matching System for Camera, monitor and printer SharpStack Plus includes: 3D Blind, 2D Blind deconvolution algorithms as well as No Neighbor, Nearest Neighbor and Inverse Filter algorithms

34. Color-Pro Module Consistent Color Better Quantitative Results ICC calibration tools for camera, screen, printer and projector

35. Evolution VF - cooled & un cooled - mono & color Features –Designed for high resolution brightfield scientific and industrial applications. –A progressive scan interline CCD sensor gives a resolution of 1.3 million pixels in a 12-bit digital output. (Sony 205 Sensor) –High-speed low noise electronics provide linear digital data –Live Frame rates of up to 110 fps with reduced region of interest and binning. –The IEEE 1394 FireWire™ digital interface allows ease of use

36. Micropublisher 3.3 Integrated Color Camera Kit High resolution 3.3 megapixel color camera Features Fully integrated with your choice of Image-Pro software FireWire (IEEE 1394) C-mount camera Max 25 fps at 640x480 (30-bit) Real Time Video (RTV) Option for cooling No interpolation or Pixel shifting Laptop kit

37. Evolution MP™ Integrated Color Camera Kit High resolution 5.1 megapixel Color Camera (Sony 282 Sensor) Features –Fully integrated with your choice of Image-Pro software –FireWire (IEEE 1394) C-mount camera –Max 25 fps at 640x480 (30-bit) Real Time Video (RTV) –Option for cooling –No interpolation or Pixel shifting –Laptop kit

38. Evolution QEi™ Integrated Camera Kit High Quantum Efficiency Camera Features –Fully integrated with your choice of Image-Pro software –FireWire (IEEE 1394) C-mount camera –Fast FireWire Live 15 fps at 1360x1036(12-bit) Ideal for demanding Lowlight and fluorescence imaging

39. Evolution Camera Series 300 400 500 600 700 800 900 1000 1100 80 70 60 50 40 30 20 10 0 Wavelength (nm) Quantum Efficiency (%) DAPI GFP TRITC CY5 IR-DIC With IR Filter (Std) Without IR Filter (Removed)

40. Evolution™ Camera Series Media Cybernetics Evolution MP 5.1 Media Cybernetics Evolution VF Media Cybernetics Evolution QEi Resolution 2560 X 1920 1392 X 1040 1360 X 1036 CCD Type ICX282 (Color/Mono) ICX205 (Color/Mono) ICX205 (Color/Mono) Sensor Grade Zero Defect Zero Defect Zero Defect Pixel Size 3.4 μm × 3.4 μm 4.65 μm × 4.65 μm 6.45 μm × 6.45 μm Full Well 8,600e- 10,000e- 18,000e-, 22,000@2x2 Read Noise 9.8e- 12e- 8e- Dark Current 2.3e-/p/s (cooled) 1e-/p/s (cooled) 0.15e-/p/s (cooled) Optical Interface 2/3” C Mount ½” C Mount 2/3” C Mount Depth 36-bit 12-bit(mono) 36-bit 12-bit(mono) Cooling 10° C below ambient 25° C below ambient 25° C below ambient Max live size 2560 X 1920 1392 X 1040 1360 X 1036 Live fps 3 fps (full) 15fps (full) @ 12-bit 15fps (full) @ 12-bit 30fps@640X480 (RTV) Binning (Max) 4X4 8X8 8X8 Gain Control 0 – 10X 0 – 15X 0 – 35X ROI ( User defined ) Yes Yes Yes RGB Filter Option Yes Yes Warranty 2 yrs 2 yrs 2 yrs

41.  Bright field, Phase- Contrast, & Darkfield Microscopy  Fluoresecence Microscopy  Live- Cell Imaging  Pathology, Histology, & Cytology  Green Fluorescent Protein (GFP) Applications  FISH  Ca++ Ratio, TIRFM, Analysis  Motility & Motion Analysis  DNA Analysis  Metallurgical Microscopy  Semiconductor Inspection  Manufacturing Quality Control  Failure Analysis  Forensic Analysis  Spinning Disk Confocal system Applications Evolution QEi™

42. Applications Evolution VF™  Brightfield, Phase- Contrast, & Darkfield Microscopy  Fluoresecence Microscopy  Live- Cell Imaging  Pathology, Histology, & Cytology  Motility & Motion Analysis  DNA Analysis  Metallurgical Microscopy  Semiconductor Inspection  Failure Analysis  Forensic Analysis

43. Applications Evolution MP5.1™  Brightfield, Phase- Contrast, & Darkfield Microscopy  Fluoresecence Microscopy  Pathology, Histology, & Cytology  Haematology  Document Imaging  Still Image Animation

44. 300 400 500 600 700 800 900 1000 1100 80 70 60 50 40 30 20 10 0 Wavelength (nm) Quantum Efficiency (%) QEi with IR Filter QEi without IR Filter DAPI GFP TRITC CY5 IR-DIC RT SE6 with IR Filter RT SE6 without IR Filter Orca ER150 CoolSNAP HQ Mono Sensicam QE LOW LIGHT Sensicam QE Standard Mode

45. Pro-Series MV-Pro & MV-Lite Capture Kits Board, Cable & Driver Included Works with Image Pro Express, Discovery or Plus Works with Most Video Cameras Specialized Cables also Available New version available soon

46. Turboscan Fast Mosaic Image Acquisition For Image-Pro® Plus Scanning and acquisition at camera frame rates Support for various analog and digital cameras, including Media Cybernetics' Evolution VF and QImaging's QICAM cameras High-quality image tiling Frame-rate shading correction Fast and accurate relocation Continuous focus tracking using predictive focus Compatible with most 3rd party motorized stage and focus drives Intelligent memory management supports mosaic sizes limited only by available disk space Plug-in capture driver for use within Image-Pro® Plus 5.1Image-Pro® Plus 5.1

47. X, Y or Z Stage Kit X,Y-axis Stage and/or Z-axis motor Controller box Use With Image Pro 5.1 MC or MDA, AMS All necessary cables

48. www.mediacy.com Silver Spring, MD (Headquarters) Offices in: Boston, MA, Pittsburgh, PA & San Diego, CA Leiden, The Netherlands Goettingen, Germany Wokingham, United Kingdom Singapore, Republic of Singapore Tokyo, Japan Shanghai, China