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Published byMabel Willis Modified over 9 years ago
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H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER FGFR2 TUSC2 Actin FGFR2 48 hours 24 hours Positive TUSC2 repressed FGFR2 expression in H1299 cells.
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TUSC2 repressed FGFR2 expression in H1650 cells but not H322 cells. FGFR2 H1650 H322 FGFR2 FGFR2 expression is very low in H322 cells Vector TUSC2 TUSC2 -ER 2.3 uM ER H1650 24 hours Vector TUSC2 TUSC2 -ER 2.3 uM ER H1650 H322 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1650
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Repeat western blotting in A549 cells FGFR2 TUSC2 Vector TUSC2 TUSC2 -ER 2.3 uM ER A549 Vector TUSC2 TUSC2 -ER 2.3 uM ER A549 Vector TUSC2 TUSC2 -ER 2.3 uM ER FGFR2 A549 24 hours 48 hours 24 hours There are two bands near the size of 120kd. TUSC2 seems repressed the upper band at 24 hours time point.
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