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Published byClementine McGee Modified over 9 years ago
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Engineering receptors and antibodies for biosensors Author: B. Hock, M. Seifert, K. Kramer Presented by: Abu Bakar Md. ISMAIL & Balakrishnan RAMANATHAN
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Objectives: 1. Effect monitoring with biological components as targets for bioeffective substances. 2. Immunochemical analysis employing anibodies as binding protiens.
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Experimental Tool Surface Plasmon Resonance (SPR) sensing principle
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Experiments Experiment 1: Characterization of receptors. Experiment 2: K inetic and affinity measurements of recombinant antibody fragments derived from antibody libraries with s-triazine selectivities.
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Experiment 1…….. Estrogen Receptor: Expression & Purification Expression of the ER in yeast (16 kDa)
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Experiment 1…….. Immobilization of Binding of ER 17ß-estradiol-BSA Estrogen Receptor: Expression & Purification Binding studies with the recombinant ER:
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Experiment 1………. Response for increasing Calibration curve for 17β-estradiol concentration Competitive receptor assay (b) Competitive receptor assay in biosensor format:
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7 minutes………...? Ooops…………... That’s all from me …………………………...
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