Download presentation
Presentation is loading. Please wait.
Published byPierce Hilary Young Modified over 9 years ago
1
Created by: Evelin Fuentes Rai Boone
2
Genetic engineering : the deliberate manipulation of genetic material. Genetic code : The sequence of nucleotides in DNA or RNA that determines the specific amino acid sequence in the synthesis of proteins.
3
It is possible to move material between species because the genetic code is universal. The transfer of genetic code can cause genetic mutations in animals
4
Gene transfer : Involves the use of plasmids, host cell (bacterium, yeast, or other cell), restriction enzymes (endonucleases), DNA ligase. Gene therapy : The treatment of certain diseases by introducing specific engineered genes in a patients cells. Vector : Is what is needed to carry the gene into the host cell; plasmids are often used as vectors. Restriction enzyme : Used to cut a desired section of the DNA. Plasmids : Used to clone a desired gene. Host cell : The cell used to receive the genetic material.
5
A human gene that codes for insulin can be inserted into a plasmid and then inserted into a host cell (bacterium). The bacterium then synthesis insulin which can then be collected and used by diabetics.
6
◦ Note- Gene transfer involves the following elements: a vector, a host cell, restriction enzyme, DNA ligase. Step 1: Introduce the desired gene into a plasmid and transfer it into a bacterial cell, then culture the bacteria. ◦ Note: many of the bacteria will have a plasmid with the desired gene Step 2: Use restriction enzymes to cut the desired gene out of the plasmids and purify the gene using gel electrophoresis.
7
Step 3:
9
"Genetic Code: Definition from Answers.com." Answers.com: Wiki Q&A Combined with Free Online Dictionary, Thesaurus, and Encyclopedias. Web. 13 Feb. 2012.. "IB Biology Notes - Genetic Engineering And Biotechnology." IB Guides - Free International Baccalaureate Study Guides, Notes, Videos and Powerpoints. Web. 13 Feb. 2012.. "Gene Therapy: Definition from Answers.com." Answers.com: Wiki Q&A Combined with Free Online Dictionary, Thesaurus, and Encyclopedias. Web. 13 Feb. 2012.. "BrainPOP | Science." BrainPOP - Animated Educational Site for Kids - Science, Social Studies, English, Math, Arts. Web. 13 Feb. 2012..
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.