1. HIV GP120 Envelope Protein
Objectives :
Observing consensus sequences of different strains
Structure analysis

2. GP120 Function
GP120 involved in infection of human T-cells

3. HIV TYPES HIV Type I SIV HIV Type II HIV Type III Human viruses
Simian virus

4. GP120 Sequence analysis
Sequence analysis shows a high mutation rate with preserved large consensus regions not only between strains, but also between different types

5. GP120 Sequence analysis HIV Type I shows additional sequence of
7 aa in it’s consensus sequence in a consensus
region in acids 27-33
consensus LWVTVFYGVPVWKEATTTLFCATDNK NTWATHACVPTDPDYQEVELNNVTENF 53
HIV LWVTVYYGVPVWHDADPVLFCASDAKahsteahNIWATQACVPTDPSPQEVFLPNVIESF 92

6. Two structures of GP120-CD4 from HIV 1 were analyzed
Hiv-1 Yu2 isolate
Hiv-1 Hxbc2 isolate

7. Difference between the proteins
The similarity is shown, using protein blast
Score = 566 bits (1460), Expect = e-161 Identities = 276/321 (85%), Positives = 289/321 (89%),
Gaps = 8/321 (2%)

8. Structure of conserved region
GP120 Hxbc2 isolate
Preserved region
marked in yellow
CD4

9. Docking of GP120 Clustered solution of Dimers With current approach
GP120 exists as a
trimers or tetramers on the membrane surface.
According to J. Biol. Chem., Vol. 275, Issue 45, , November 10, 2000

10. Conclusions Preserved sequences found
GP120-CD4 theoretical docking performed, clustered solutions found
The results provide a basis for further research