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Bacterial Motility Hugh B. Fackrell. 2 8/5/2015 Presentation Outline l Bacterial Flagella l Location l Function l Structure l Bacterial vs eukaryotic.

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Presentation on theme: "Bacterial Motility Hugh B. Fackrell. 2 8/5/2015 Presentation Outline l Bacterial Flagella l Location l Function l Structure l Bacterial vs eukaryotic."— Presentation transcript:

1 Bacterial Motility Hugh B. Fackrell

2 2 8/5/2015 Presentation Outline l Bacterial Flagella l Location l Function l Structure l Bacterial vs eukaryotic flagella

3 3 8/5/2015 Flagellum vs Pilus Pilus Flagellum

4 4 8/5/2015 Pilus (fimbriae) l An appendage of the bacterial cell used for transfer of genetic information l Plural pili

5 5 8/5/2015 Pili Transfer Plasmids

6 6 8/5/2015 Bacterial Flagellum l A thin appendage arising from one or more locations on the surface of the cell wall l Cellular locomotion l Moves cell along gradients e.g. glucose

7 7 8/5/2015 Types of Motility l Flagellated –Flagellum- l coordinated movement l tumbling movement –Axial filament- spirochetes l Gliding- no visible organelles

8 8 8/5/2015 Location l Polar l Subpolar l Lateral l Lophotrichous l Amphitrichous l Peritrichous l Periplasmic

9 9 8/5/2015 Polar Flagellum

10 10 8/5/2015 Lophotrichous Flagella Spirillum volutns two or more flagella at the end of the microbe Photographs show growth of flagella during replication

11 11 8/5/2015 Amphitrichous Flagella l Tufts of flagella at BOTH ends of the microbe

12 12 8/5/2015 Peritrichous Flagella

13 13 8/5/2015 Flagellar tufts

14 14 8/5/2015 Flagellar tufts

15 15 8/5/2015 Spirochetes have Periplasmic Flagella l Periplasmic flagella are also called the axial filament

16 16 8/5/2015 Axial filaments

17 17 8/5/2015 Bacterial Flagellum

18 18 8/5/2015 Rings of Gram Negative Flagellum l M: cell Membrane l P: Peptidoglycan l L: Outer Layer l S: Spacer

19 19 8/5/2015 Flagellar Structure (details)

20 20 8/5/2015 Prokaryotic Flagellum

21 21 8/5/2015 Flagellar Structures

22 22 8/5/2015 Assembly of Flagellum

23 23 8/5/2015 Demonstration of Chemotaxis l Capillary tube contains attractant chemical l Attractants: –Glucose,Maltose, Alanine l Repellents: –Indole, H+, OH-, fatty acids

24 24 8/5/2015 Flagellar Rotation l Clockwise: Tumbling l Counterclockwise: coordinated l Alternate between two rotations

25 25 8/5/2015 “Run & Tumble” motility

26 26 8/5/2015 Regulation of Flagellar Movement

27 27 8/5/2015 Movement of Flagella l Whip l Egg beater

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