1. CHEM 210 PRESENTATION FALL 2013 PROFESSOR: WAN JIN JAHNG PhD
CHEM 210 PRESENTATION FALL PROFESSOR: WAN JIN JAHNG PhD. By JOHNPAUL OFFOR (JAYPEE)

2. HYPOTHESIS -CANCER CURE LIES IN GRAVIOLA TREE (SOUR SUP) -ANNONACIN FROM GRAVIOLA TREE CAN INHIBIT MULTI-DRUG RESISTANT CANCEROUS CELLS IN HUMANS.

3. SPECIFIC AIMS - Using annonacin from graviola tree to eliminate multi-drug resistant tumor/cancerous cells - Fighting against multi-drug resistant cancerous cells -To extract pure sample of annonacin from seeds of graviola tree (sour sop) - Ensuring cancer free environment.

4. Annonacin is present in the leaves, fruits, stems and seeds of graviola trees.
Make sour- sop juice your best friend!!!

5. INNOVATION
-Most of the cancerous cells are becoming more resistance to cancer drugs.
-Increase in the number of cancer patients annually.
-Elimination of Multi-drug resistant cancerous cells.
-To make our environment cancer free.

6. SIGNIFICANCE
-Annonacin has the ability to inhibit multi-drug resistance cancerous cells.
- It can eliminate cancer multi-drug resistance cells more faster than other cancer drugs.

7. Annonacin - class of compounds known as acetogenins
Annonacin - class of compounds known as acetogenins. - characterized by linear 35carbon chains containing oxygenated functional groups including hydroxyls, ester, epoxides Formula: C35H64O7

8. Extraction and purification of annonacin -dried and powdered seed (671 g) of A. muricata were extracted with methanol at room temperature (20C) to give a brown extract (60 g). - concentrated methanol extract was partitioned between dichloromethane and water to yield 45 g of dichloromethane extract, that reacted positively with the Kedde reagent (10% 3,5-dinitho- benzoic acid in ethanol, followed by 2 N KOH in methanol), indicating the presence of unsaturated lactones. -This extract was subjected to silica gel column chromatography (silica gel 60 Merck, 70–230 mesh) and was eluted with n-hexane containing gradually increasing amounts of ethyl acetate.

9. CONT’D -The fractions collected were analysed by TLC (silica gel Merck 60 F254), on the basis of which they were grouped into 17 pooled fractions. -The solvent from fraction 15 was removed, and the resulting residue (12.2 g, Kedde positive) was further subjected to column chromatography (silica gel 60 H Merck; mobile phase dichloromethane/ isopropanol 90/10) to yield crude annonacin (840 mg). -The purity of this compound was confirmed by an analytical HPLC procedure using a reversed-phase Waters, Pure annonacin was confirmed.

10. MECHANISM OF ACTION OF ANNONACIN
-Multi-drug resistant cancerous cells form pump
-Blocking of larger pumps formed by cancerous cells
-These pumps are called P-glycoprotein

12. References
Champy, Pieire and at al. “Annonacin, a lipophilic inhibitor of mitochondrial complex I, induces nigral and striatal neurodegeneration in rats: possible relevance for atypical parkinsonism in Guadeloupe.” Journal of Neurochemistry (2008): Document.
Postl, Marqie. Graviola Shows Promise in Fighting Drug-Resistant Tumors. 12 November Web. 20 October 2013.
Taylor, Leslie. “Technical Data Report for Graviola (Annonacin).” Sage (2010): Print.