1. Osler Institute – Pathology Board Review: Electron Microscopy EM of Tumors and Tumor-Like Entities

2. Osler Institute – Pathology Board Review: Electron Microscopy Acinar Cell Carcinoma Zymogen granules 0.3 – 1.5 microns Stacks of RER Salivary gland Pancreas Pancreaticoblastoma Immunopositive for alpha-1 antitrypsin Trypsin Chymotrypsin Lipase

3. Adenocarcinoma – extracellular lumina, intestinal type microvilli Osler Institute – Pathology Board Review: Electron Microscopy

4. Neoplasms Adenocarcinoma Intracytoplasmic lumina Microvilli Junctional complexes

5. Neoplasms Osler Institute – Pathology Board Review: Electron Microscopy

6. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Bronchioloalveolar Cell carcinoma Type II Pneumocyte Myelin figures Microvilli Clara Cells Secretory granules

7. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Carcinoid (Neuroendocrine) Tumors Dense core granules 140 – 400 nm True lumens Intracytoplasmic filaments

8. Electron Microscopy - Osler Institute Pathology Board Review Eosinophilic Granuloma Langerhan’s cell histiocyte Rod shaped Birbeck Granule Pleomorphic, deeply cleaved nucleus

9. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Ependymoma 5 – 7% of intracranial neuroglial neoplasms 60% of neuroglial tumors in spinal cord Most common sites – fourth ventricle and lumbosacral region Flexner type rosettes True lumens – cilia, microvilli and junctional complexes

10. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Ependymoma Junctional complexes Intracytoplasmic lumina Intracytoplasmic glial filaments Immunopositive for GFAP

11. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Granular Cell Tumor Pleomorphic secondary lysosomes Basal lamina around cell groups

12. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Granular Cell Tumor Angulate lysosomes in stromal fibrohistiocytic cells

13. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Hibernoma Lipid droplets Electron dense mitochondria S-100 protein + Vimentin +

14. Electron Microscopy - Osler Institute Pathology Board Review Lymphoma Non Hodgkin's Type No junctional complexes Few cytoplasmic organelles Abundant polyribosomes

15. Electron Microscopy - Osler Institute Pathology Board Review Lymphoma Non Hodgkin's Type No junctional complexes Few cytoplasmic organelles Abundant polyribosomes

16. Electron Microscopy - Osler Institute Pathology Board Review Neoplasms Mesotheliomas Long thin microvilli Bipolar cytoplasmic processes Desmosomes Primitive junctions Calretinin + Mesothelin +