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Lysogeny: Phage Entry into Bacterial Hosts Bobby Chaggar Yordanos Teferra.

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Presentation on theme: "Lysogeny: Phage Entry into Bacterial Hosts Bobby Chaggar Yordanos Teferra."— Presentation transcript:

1 Lysogeny: Phage Entry into Bacterial Hosts Bobby Chaggar Yordanos Teferra

2

3 attP and attB regions attP site of L5 phage used in comparing phages and similar attB site of M. Smegmatis used in finding other att sites.

4 Search for Similar attP regions

5 Search for similar attB sites M. Segmatis: GCGGGCGTAGCTCAATGGTAGAGCCCTAG TCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTCGCCCGCTC Msme-155.Msmeg-11642Msme-155.Msmeg-11642 (4764496 -> 4764566) Aliases:(tRNA-Gly”) M. Vanbaalenii: GCGGGCGTAGCTCAATGGTAGAGCCCTAG TCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTCGCCCGCTCCA Mvan-PYR-1.Mvan-PYR-1-10996 Mvan-PYR-1.Mvan-PYR-1-10996 (4324373 -> 4324446) Aliases:(tRNA-Gly”) M. SP.JLS: GCGGGCGTAGCTCAATGGTAGAGCCCTAGTCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTC GCCCGCTCCA M-JLS.Mjls-9576 M-JLS.Mjls-9576 (3780254 -> 3780327) Aliases:(tRNA-Gly”)

6 The Search for Prophage DNA in Mycobacteria

7 Sliding Window

8 Deviations in GC content

9 Proteins to Consider Tail tape measure protein Phage major capsid protein Large-subunit terminase Integrase excisionase

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11 References Gomathi, N.S.. "In silico analysis of mycobacteriophage Che12 genome: Characterization of genes required to lysogenise Mycobacterium tuberculosis." ScienceDirect. 31. (2007): 82-91. Web. Hatful, Graham. "Characterization of the Mycobacteriophage L5 Attachment Site, attp." JMB. 266. (1997): 76-92. Canchaya, C., Proux, C., Fournous, G., Bruttin, A., & Brussow, H. (2003). Prophage genomics. Microbiol Mol Biol Rev. 2003 June; 67(2): 238–276. doi: 10.1128/MMBR.67.2.238-276.2003 Hendrix, R., Smith, M., Burns, R., Ford, M., & Hatfull, G. (1999). Evolutionary relationships among diverse bacteriophages and prophages: All the world’s a phage. Proc Natl Acad Sci U S A. 1999 March 2; 96(5): 2192–2197. Evolution. Karlin, S. (2001). Detecting anomalous gene clusters and pathogenicity islands in diverse bacterial genomes. TRENDS in Microbiology Vol.9 No.7 July 2001


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