Download presentation
Presentation is loading. Please wait.
Published byThomasine Hodge Modified over 9 years ago
1
Lysogeny: Phage Entry into Bacterial Hosts Bobby Chaggar Yordanos Teferra
3
attP and attB regions attP site of L5 phage used in comparing phages and similar attB site of M. Smegmatis used in finding other att sites.
4
Search for Similar attP regions
5
Search for similar attB sites M. Segmatis: GCGGGCGTAGCTCAATGGTAGAGCCCTAG TCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTCGCCCGCTC Msme-155.Msmeg-11642Msme-155.Msmeg-11642 (4764496 -> 4764566) Aliases:(tRNA-Gly”) M. Vanbaalenii: GCGGGCGTAGCTCAATGGTAGAGCCCTAG TCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTCGCCCGCTCCA Mvan-PYR-1.Mvan-PYR-1-10996 Mvan-PYR-1.Mvan-PYR-1-10996 (4324373 -> 4324446) Aliases:(tRNA-Gly”) M. SP.JLS: GCGGGCGTAGCTCAATGGTAGAGCCCTAGTCTTCCAAACTAGCTACGCGGGTTCGATTCCCGTC GCCCGCTCCA M-JLS.Mjls-9576 M-JLS.Mjls-9576 (3780254 -> 3780327) Aliases:(tRNA-Gly”)
6
The Search for Prophage DNA in Mycobacteria
7
Sliding Window
8
Deviations in GC content
9
Proteins to Consider Tail tape measure protein Phage major capsid protein Large-subunit terminase Integrase excisionase
11
References Gomathi, N.S.. "In silico analysis of mycobacteriophage Che12 genome: Characterization of genes required to lysogenise Mycobacterium tuberculosis." ScienceDirect. 31. (2007): 82-91. Web. Hatful, Graham. "Characterization of the Mycobacteriophage L5 Attachment Site, attp." JMB. 266. (1997): 76-92. Canchaya, C., Proux, C., Fournous, G., Bruttin, A., & Brussow, H. (2003). Prophage genomics. Microbiol Mol Biol Rev. 2003 June; 67(2): 238–276. doi: 10.1128/MMBR.67.2.238-276.2003 Hendrix, R., Smith, M., Burns, R., Ford, M., & Hatfull, G. (1999). Evolutionary relationships among diverse bacteriophages and prophages: All the world’s a phage. Proc Natl Acad Sci U S A. 1999 March 2; 96(5): 2192–2197. Evolution. Karlin, S. (2001). Detecting anomalous gene clusters and pathogenicity islands in diverse bacterial genomes. TRENDS in Microbiology Vol.9 No.7 July 2001
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.