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Blood Start
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TABLE OF CONTENTS Blood Blood Components Blood Typing
Blood Cell Differential Identification Blood Cell Counting Using a Hemocytometer Blood
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BLOOD TYPING Blood ABO Agglutination Result Rh Agglutination Result
Type A Type AB Rh + Type B Type O Rh - Antigen – Antibody Composition Blood Type Compatibility Comparisons Blood
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ABO Blood Typing Type A Type B Type AB Type O Blood
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Type A Agglutination Result
Note the appearance of fine granulation (pepper grains) in the blood No Agglutination Note the lack of granulation in the blood Blood
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Type B Agglutination Result
No Agglutination Agglutination Blood
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Type AB Agglutination Result
Blood
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Type O Agglutination Result
No Agglutination No Agglutination Blood
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Rh Blood Typing Rh+ Rh- Blood
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Rh+ Agglutination Result
Blood
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Rh- Agglutination Result
No Agglutination Blood
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BLOOD CELL DIFFERENTIAL IDENTIFICATION
Human Blood Smear 400x Erythrocytes 400x 1000x Leukocytes Cell Types Thrombocytes 400x 1000x Blood
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Human Blood Smear 400x erythrocyte thrombocyte leukocyte Blood
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Erythrocytes 400x Blood
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Erythrocytes 1000x Blood
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LEUKOCYTES Blood Neutrophil Eosinophil Basophil Lymphocyte Monocyte
1000x Eosinophil 1000x Basophil 1000x Lymphocyte 1000x Monocyte 1000x Blood
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Leukocyte - Neutrophil 1000x
thrombocyte erythrocyte neutrophil Blood
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Leukocyte - Eosinophil 1000x
erythrocyte eosinophil thrombocyte Blood
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Leukocyte - Basophil 1000x erythrocyte basophil thrombocyte Blood
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Leukocyte - Lymphocyte 1000x
thrombocyte lymphocyte erythrocyte Blood
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Leukocyte - Monocyte 1000x erythrocyte monocyte thrombocyte Blood
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Thrombocytes 400x thrombocytes Blood
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Thrombocytes 1000x thrombocytes Blood
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BLOOD CELL COUNTING USING A HEMOCYTOMETER
Microvolume Pipetters Hemocytometer Loading the Hemocytometer Hemocytometer Grid (Unloaded) 40x Hemocytometer Grid (Unloaded) 100x Hemocytometer Grid (Unloaded) 400x Hemocytometer Grid (Loaded) 100x Hemocytometer Grid (Loaded) 400x Blood
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Microvolume Pipetters
Blood
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Hemocytometer grid grid Blood
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Loading Hemocytometer
pipet tip cover slip Blood
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Hemocytometer Grid (Unloaded) 40x
1 2 3 4 5 RBC counted in 5 numbered areas Blood
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Hemocytometer Grid (Unloaded) 100x
3 Area 3 of previous magnification Blood
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Hemocytometer Grid (Unloaded) 400x
Blood
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Hemocytometer Grid (Loaded) 100x
loaded with RBC Blood
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Hemocytometer Grid (Loaded) 400x
inside of each box, cell not counted if on bottom line or on right line cell not counted cell counted inside of each box, cell counted if within box, on top line or on left line Blood
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Blood separate from the lighter, liquid components (plasma)
When whole blood is spun down in a centrifuge, the heavier, solid component (cells) … Blood
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click on a blood component
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PLASMA (~55%) includes water, proteins, electrolytes, antibodies, organic nutrients and organic wastes Blood
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ERYTHROCYTES (~45%) Blood
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“BUFFY COAT” (~1%) LEUKOCYTES and THROMBOCYTES
Blood
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ANTIGEN-ANTIBODY COMPOSITION
TYPE A Rh+ TYPE B Rh- TYPE AB TYPE O Blood
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Type A Whole Blood Antibody Antigen Antigen Antibody A b RBC Blood
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Type B Whole Blood Antibody Antigen Antigen Antibody B a RBC Blood
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Antigens Type AB Whole Blood Antibodies Antigen A Antigen B RBC Blood
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a b Blood Type O Whole Blood B RBC Antigens Antibodies Antibody
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Blood Rh+ Whole Blood B a RBC Antigen Antibody Antibody Rh+ Antigen
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Antigen Rh- Whole Blood Antibody Antigen Antibody B a RBC Blood
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BLOOD TYPE COMPATIBILITY COMPARISONS
Type A RBC Type B RBC Type A plasma Type AB plasma Type A plasma Type AB plasma Type B plasma Type O plasma Type B plasma Type O plasma Type AB RBC Type O RBC Type A plasma Type AB plasma Type A plasma Type AB plasma Type B plasma Type O plasma Type B plasma Type O plasma Blood
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A b Blood RBC Agglutination NO Hemolysis NO Compatible Type A RBC
crossed with Type A Plasma Antigen Antibody A b RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a A Blood a RBC Agglutination YES Hemolysis YES Compatible Type A RBC
crossed with Type B Plasma Antigen Antibody Antibody a a A RBC Agglutination YES Hemolysis YES Compatible NO Blood
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A Blood RBC Agglutination NO Hemolysis NO Compatible Type A RBC
crossed with Type AB Plasma Antigen A RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a A b Blood a RBC Agglutination YES Hemolysis YES Compatible
Type A RBC crossed with Type O Plasma Antigen Antibody Antibody a a A Antibody b RBC Agglutination YES Hemolysis YES Compatible NO Blood
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B a Blood a RBC Agglutination NO Hemolysis NO Compatible Type B RBC
crossed with Type B Plasma Antigen Antibody Antibody B a a RBC Agglutination NO Hemolysis NO Compatible YES Blood
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B b Blood a RBC Agglutination YES Hemolysis YES Compatible Type B RBC
crossed with Type A Plasma Antigen Antibody Antibody B a b RBC Agglutination YES Hemolysis YES Compatible NO Blood
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B Blood a RBC Agglutination NO Hemolysis NO Compatible Type B RBC
crossed with Type AB Plasma Antigen Antibody B a RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a B b Blood a RBC Agglutination YES Hemolysis YES Compatible
Type B RBC crossed with Type O Plasma Antibody Antigen Antibody a B a Antibody b RBC Agglutination YES Hemolysis YES Compatible NO Blood
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A b B Blood A B RBC Agglutination YES Hemolysis YES Compatible
Type AB RBC crossed with Type A Plasma Antigen Antigen Antibody A A b Antigen Antigen B B RBC Agglutination YES Hemolysis YES Compatible NO Blood
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a A B Blood A B RBC Agglutination YES Hemolysis YES Compatible
Type AB RBC crossed with Type B Plasma Antigen Antigen Antibody A a A Antigen Antigen B B RBC Agglutination YES Hemolysis YES Compatible NO Blood
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A B Blood A B RBC Agglutination NO Hemolysis NO Compatible Type AB RBC
crossed with Type AB Plasma Antigen Antigen A A Antigen Antigen B B RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a A b B Blood A B RBC Agglutination YES Hemolysis YES Compatible
Type AB RBC crossed with Type O Plasma Antigen Antigen Antibody a A A Antibody Antigen Antigen b B B RBC Agglutination YES Hemolysis YES Compatible NO Blood
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b Blood B a RBC Agglutination NO Hemolysis NO Compatible Type O RBC
crossed with Type A Plasma Antigen Antibody Antibody B a b RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a Blood B a RBC Agglutination NO Hemolysis NO Compatible Type O RBC
crossed with Type B Plasma Antigen Antibody Antibody B a a RBC Agglutination NO Hemolysis NO Compatible YES Blood
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Blood B a RBC Agglutination NO Hemolysis NO Compatible Type O RBC
crossed with Type AB Plasma Antigen Antibody B a RBC Agglutination NO Hemolysis NO Compatible YES Blood
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a b Blood B a RBC Agglutination NO Hemolysis NO Compatible Type O RBC
crossed with Type O Plasma Antigen Antibody Antibody a B a Antibody b RBC Agglutination NO Hemolysis NO Compatible YES Blood
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