1. POROS® Affinity Columns Protein A, Protein G, and CaptureSelect® Affinity Columns
Intended for analytical scientists supporting the development of biologic drugs.
Or for small-scale purification of immunoglobulins, Fabs, or fusion proteins.
POROS® Affinity Columns enable:
Direct sampling from cell culture or fermentation harvests
Welcome to this short presentation that is intended to provide a brief overview of the Poros Affinity Column range
These prepacked Columns are intended for analytical scientists supporting the development of biologic drugs that require an assay to quantitate proteins produced in cell culture or fermentation harvests. They are also suitable for small scale purification of Immunoglobulins, Fabs, or Fusion proteins.
POROS® CaptureSelect® HPLC columns incorporate the advantages of high-speed and high performance POROS® chromatography resins with the unique selectivity of Protein A, G and CaptureSelect ligands. The combination allows precise, three minute quantitation.
In Addition the eluted product is highly purified and suitable for further analysis including mass Spectrometry
Precise quantitation of product titers, typically in under 3 minutes
with the eluted product being highly purified and suitable for further analysis including mass spectrometry.

2. POROS® Affinity Columns Prepacked column overview
Product Name
Specificity
Dynamic Binding Capacity (mg/mL)
POROS® A 20
Human, mouse, and 5 other species
19.0
POROS® G 20
Human, mouse, rat, and 8 other species
9.0
POROS® CaptureSelect® IgG Fc
Human
8.9
POROS® CaptureSelect® IgM
Human, mouse, and rat 4
POROS® CaptureSelect® LC Kappa
Human, all subclasses
14.5
POROS® CaptureSelect® LC Lambda 10
POROS® CaptureSelect® HSA
2.6
POROS® prepacked columns
Column Dimension
Diameter (mm) x Length (mm)
Column Volume
(mL)
2.1 x 30
0.1
4.6 x 50
0.8
4.6 x 100
1.7
10 x 100
7.9
The Poros Prepacked Affinity Column range now includes 7 ligands immobilized on our Poros 20 µm beads and are offered in the four prepacked column sizes shown in the table to the right
In the table to the left, the specificities of Protein A and G, shown in the top rows, are well documented.
The Poros CaptureSelect range is the result of a Life Technologies and BAC collaboration. Unique specificities include:
Poros®CaptureSelect®IgG-Fc which allows for the detection of any human IgG subclass (even IgG3) in the presence of IgG from other sources like bovine.
Poros®CaptureSelect®IgM can be used for the analysis of human, mouse and rat IgM
Poros®CaptureSelect®LC-kappa and Poros®CaptureSelect®LC-lambda products allow for the analysis of human antibodies and antibody fragments containing either a kappa or a lambda light chain.
Poros®CaptureSelect®HSA product is well suited for analysis of HSA-fusion proteins and is specific for human serum albumin allowing for use even in the presence of albumin from other species.
POROS® CaptureSelect® Columns
A collaboration between Life Technologies and BAC

3. POROS® Affinity Columns Protein A, Protein G, and CaptureSelect® specificity
Kappa or Lambda
IgG Fc
CH3
CH1
CH2 VH CL VL
Protein A or Protein G
POROS® Protein A & Protein G resins
POROS® CaptureSelect® ligands
Bimolecular type
Protein A
Protein G
IgG Fc
Kappa
Lambda
HSA
IgM
IgG1, IgG2, IgG4 
IgG3
IgG, CH2 domain deleted
IgA, IgD, IgE
Fab, Fab'2
Human serum albumin
Albumin fusion protein
IgG Fc fusion protein
A comparison of Protein A, G and Capture Select binding specificities is shown in the table and is also included in the Poros Affinity Column Product Bulletin
The Antibody cartoon shows the location of binding epitopes for Protein A, G and the CaptureSelect ligands. Note that in situations where modifications to an antibody’s Fc region impact Protein A or G binding, the CaptureSelect IgG Fc product may provide a solution for quatitation or purification.
The CaptureSelect Kappa and Lambda ligands both bind to the constant region of their respective light chains and bind all subclasses. In comparison, Protein L binds the VL domain of the Kappa light chain and exhibits strong affinity for 4 of the 7 subclasses.
POROS® Protein A, Protein G, and CaptureSelect® ligands target multiple IgG epitopes. The graphic shows the binding sites for affinity ligands Protein A and Protein G. Novel POROS® CaptureSelect® ligands provide alternative specificities that enable rapid binding for the quantitation or purification of engineered immunoglobulins.

4. POROS® Affinity Columns Additional information
Product Bulletin: POROS® Protein A, Protein G, and CaptureSelect® Columns
Application Note: POROS® A 20 Analytical HPLC Columns
Operating Instruction: POROS® Protein A, Protein G and CaptureSelect® Affinity Columns
Additional information on performance and product specifications may be found in the product bulletin. The Poros A 20 and Operating Instructions publications provide product use details. Lastly, for more information on BAC’s product range please visit their web site.
I hope this introduction was helpful and should you have questions please contact us at
Thanks for your time today
Go to BAC: bacbv.com
© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. CaptureSelect is a registered trademark of BAC BV.