1. BIOLOGYofCOMPLEMENT

2. BIOLOGYDIAGNOSIS COMPLEMENT

3. =C4d

4. = C3injury

5. = C4d C3injury MBL Apoptotic CMV cells CRP

6. = C4d C3injury MBL Apoptotic CMV cells CRP Regulators

7. = C4d C3injury MBL Apoptotic CMV cells CRP Regulators protection

8. C1 C4 C2 C3 MBL B C5 Classical Lectin Alternative Three Pathways of Complement Activation

9. C1 C4 C2 C3 MBL B C5 Classical Lectin Alternative Three Pathways of Complement Activation a a a b b b b a

10. C1C2C5C6C7C8C9 C4 C3 0.6 mg/ml 1.3 mg/ml C4bC2a C3bBb

11. C1C2C5C6C7C8C9 C4 C3 0.6 mg/ml 1.3 mg/ml C1inh Factor I C4bp CR1 MCP Factor I DAF CR1 MCP C4bp Factor I DAF CR1 Factor H CD59 Clusterin Vitronectin C4bC2a C3bBb

15. C3b > C4b CR1 iC3b > iC4b CR3 C3d > C4d CR2 FUNCTION

16. C4d vs C3d STAINING

17. ATGAM Thymoglobulin (Horse polyclonal) (Rabbit polyclonal) C4d+ / Total C4d Deposition ATGAM vs Thymoglobulin 9 / 9 0 / 8

19. C Reactive Protein Activation of Complement

21. ANTIBODY ASSOCIATED REJECTION 7 years after transplantation anti-DR7 and DRw53

22. ANTIBODY ASSOCIATED REJECTION after plasmapheresis plus IVIg anti-DR7 and DRw53 undetectable C4dC3d

23. Clearance of iC3b and C3d from Human Endothelial Cells in vitro

24. “SUBLYTIC” MAC LEUKOCYTE ACTIVATION BY

25. MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC W-P

26. MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC P-selectin vWfCD63 Tissue Factor

27. MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC P-selectin vWfCD63 Tissue Factor Synthesis PAF MCP-1 bFGF P-selectin Tissue Factor PDGF IL-8

28. MAC Endothelial P-selectin vWfCD63 Tissue Factor Synthesis PAF MCP-1 bFGF P-selectin Tissue Factor PDGF IL-8 Monocyte/ Macrophage Patelet P-selectin bFGF PDGF vWf Tissue Factor TNF 

32. B. Wasowska et al 2001 Transplantation 71, 727. vWf

33. Ig Knock Out Recipients

34. Tan et al. Arth & Rheum 40: 1601-1611, 1997 1:3201:1601:801:40 Operating Characteristics of ANA Immunofluorescence Assays

35. HUMORAL IMMUNITY IN CARDIAC TRANSPLANTS Cardiac Surgery: J. Conte Cardiology: D Carter, EK Kasper, D Judge Immunogenetics: D Lucas, MS Leffell, AA Zachary Bioengineering: J Haun, R Alevriadou Pathology: RH Hruban, ER Rodriguez, WM Baldwin III