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PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph.

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Presentation on theme: "PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph."— Presentation transcript:

1 PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph.

2 History of question was described for the first time by Selby at 1903 The 60 strains of this virus are infecting about 500 plant species from 67 families in nature CMV strains are divided into two groups according to the differences in their genomes and biological features More than 40 isolates was described at Primorye region On the base of serological assay isolates from Primorye were ascribed to Far Eastern serotype at 2008 orchid isolates was ascribed to Eastern Asian isolates

3 Aim The aim of our work is to show are the examined orchid isolates came from abroad or they are belonging to natural Far-Eastern CMV population

4 Virus structure spherical form size ~29 nm 32 morphological subunits of coat protein in nodes of crystal lattice contain 3 molecules of (+) RNA

5 Methods Infected plant Total RNA Sequencing Nicotiana tobacum cv Xanthi by Dreyper (1991) Isolation RT-PCR primersequence 5`-3`lenght, b Annealing temp. °C Length of product, bp FATGGAATTGAACGAAGGCGC1958500 RTGGGCGGAAGGGTCATTTTGA20 was made by “Sintol” company

6 Phylogenetic analysis South Korea China India China Far-Eastern China East-Asian isolates

7 Genetic distances JpSKEurChIndUSAFE Jp SK0.141 Eur0.3030.290 Ch0.2550.2160.336 Ind0.2290.1630.3390.190 USA0.1660.0770.3020.1970.148 FE0.2040.1270.3270.1730.1120.120 Outgroup1.0691.0491.1701.0590.9801.0431.063 Jp – Japan, SK – South Korea, Eur – Europe, Ch – China, Ind – india, FE – Far-Eastern isolates

8 At present time we have separate population of CMV on orchid plants on Far-Eastern territory of Russian Federation. On both genetic distances and phylogenetic reconstruction Far-Eastern isolates are part of East-Asian Isolates. Conclusion

9 Thanks for your attention

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