1. Raman spectroscopy

2. Mr/ Abd El mo’ez Ahmed Mohammed “3th group” “Neutron physics and application in material science and nuclear application” physics department Faculty of Science Sohag University Egypt Faculty of Science Physics Department

3. JINR’s group Supervisor : prof. S.I. Tutunnikov. Technician : Mr. Kovalev Yura.

4. Why we use Raman spectroscopy? Raman spectroscopy is used to determine the molecular motions, especially the vibrational one.

5. Now what is the application? We can use Raman spectroscopy in:- Tissue characterization. Tissue characterization. Tissue Imaging. Tissue Imaging. Live Cell Imaging. Live Cell Imaging.

6. Tissue characterization Tissue characterization

7. Tissue Imaging

8. Live Cell Imaging All the sexiness, without the dyes!!

9. h h(  -+) 1 ) h 3 2 1 0 S 1 3 2 1 0 S 0 Energy Virtual Level Rayleigh Raman (inelastic) (elastic) Scattering Scattering Inelastic Scattering difference in energy Energy transferred from incident light to molecular vibrations

10. Raman Spectrometer Main view of SOLAR-TII setup

11. Raman spectrometer’s mechanism. The laser beam falls on the filter and then it passes to the objective and collides with the Sample. The light will be scattered in all directions and will be reflected to the objective again. After the reflected light reaches the objective it falls down on the spectrometer system and finally to the CCD or PMT detectors.

12. Principle of Confocal Microscopy

13. Experimental data of a ceramic material.

14. Acknowledgement Finally I’d like to thank 1- Mr/ Kovalev Yura. 2- Mr/ Valantine. for their help and efforts.